CD155 Monoclonal Antibody (2H7CD155), APC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 2H7CD155 recognizes CD155 also known as the poliovirus receptor
• The CD155 protein is a member of the Ig superfamily in which 4 isoforms exist; only the alpha and delta forms are expressed on the membrane of monocytes, prostate cancer cells, as well as neurectodermal and monocytic tumor cells lines
• In addition, CD155 is found during mid-gastrulation during embryonic development
• CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells
• CD226 and CD96 are ligands for CD155
• Blocking of CD155 is under therapeutic investigation for prevention of variants of poliovirus
• Applications Reported: This 2H7CD155 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 2H7CD155 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells or the human tumor cell line U937
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily
• The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1
• The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication
• Multiple transcript variants encoding different isoforms have been found for this gene.