CD307d (FcRL4) Monoclonal Antibody (413D12), APC, eBioscience™, Invitrogen™

Description

• Description: The 413D12 monoclonal antibody reacts with human CD307d, which is also known as FcRL4, Fc receptor-like 4, FcRH4, IRTA4, or IGFP2
• CD307d is an inhibitory receptor that is highly expressed on a subset of memory B cells resident in epithelial tissues and marginal zones of mucosal lymphoid tissues such as tonsils
• CD307d has been shown to recruit the phosphatase SHP-1, thereby acting as an inhibitory receptor in B cells
• CD307d is homologous to FcgammaRI and specifically binds IgA
• To date, CD307d is the only known inhibitory receptor for IgA
• Expression of CD307d is increased on memory B cells from patients with chronic infections such as HIV, suggesting that enhanced expression of inhibitory receptors, such as CD307d, may contribute to the inefficiency of antibody responses against HIV
• Applications Reported: This 413D12 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 413D12 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells and transfected 293T cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1
• The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs
• This protein may play a role in the function of memory B-cells in the epithelia
• Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma.