Phospho-CD247 (CD3 zeta) (Tyr142) Monoclonal Antibody (3ZBR4S), APC, eBioscience™, Invitrogen™

Description

• This 3ZBR4S monoclonal antibody recognizes human and mouse CD247 (CD3z) when phosphorylated on Tyrosine 142
• CD3 is a multi-protein complex expressed on the surface of thymocytes and T cells, a marker of the T cell lineage, and a critical component of T cell receptor (TCR) signaling
• The complex contains a gamma chain, a delta chain, and two epsilon chains, which associate with the alpha/beta or gamma/delta chains of the TCR, and the zeta chain homodimer
• The CD3 zeta chain is responsible for initiating the signaling cascade following engagement of the TCR
• Each CD3 zeta chain contains three immunoreceptor tyrosine-based activation motifs (ITAM) that are phosphorylated by Src kinases such as Lck
• Once phosphorylated, these ITAMs form docking sites for SH2 domain-containing proteins and function to recruit ZAP-70 to the TCR where it can further amplify and propagate signaling
• Loss or mutation of CD3 zeta or the ITAM motifs results in impaired T cell development, signaling, and function
• CD3 zeta phosphorylation occurs early in the TCR signaling cascade
• It occurs immediately downstream of Lck activation, and upstream of ZAP-70, LAT, and SLP-76
• Applications Reported: This 3ZBR4S antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3ZBR4S antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex
• T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta
• These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11
• The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits
• CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation
• The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum
• It is never expressed on B or NK cells
• Decreased percentages of T lymphocytes may be observed in some autoimmune diseases
• Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.