Amphiregulin Monoclonal Antibody (AREG559), APC, eBioscience™, Invitrogen™

Description

• Description: The AREG858 monoclonal antibody reacts with human Amphiregulin (AR)
• AR is a member of the Epidermal Growth Factor (EGF) family and is expressed as a transmembrane precursor protein and released by proteolytic cleavage
• Soluble AR is a ligand for the EGF receptor (EGFR) and the interaction promotes growth of normal epithelial cells, fibroblasts and keratinocytes
• In the context of the immune system AR functions as an effector molecule for tissue repair and homeostasis, mediates resistance and tolerance to infection, and suppresses inflammation
• Under different disease and activating conditions AR is expressed in T cells, group 2 innate lymphoid cells, basophils, mast cells, eosinophils, and neutrophils
• Applications Reported: This AREG559 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This AREG559 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF
• The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor
• Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR
• These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin
• All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds
• AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta
• AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.