MHC I Free Chain Monoclonal Antibody (A4), APC, eBioscience™, Invitrogen™

Description

• Description: The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of beta2 microglobulin
• There are three class I alpha-chain genes in humans, called HLA-A, -B and -C
• Normally, HLA class I proteins are associated non-covalently with beta2 microglobulin (beta2M)
• The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells
• Class I HLA is expressed by the majority of nucleated cells
• Applications Reported: This A4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This A4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• MHC I Free Chain Monoclonal antibody specifically detects MHC I Free Chain in Human samples
• It is validated for Flow Cytometry.