E-Cadherin Monoclonal Antibody (4A2C7), Invitrogen™

Description

• This monoclonal antibody reacts with human epithelial cadherin (E-cadherin, uvomorulin, Cell-CAM 120/80), a 120 kDa transmembrane glycoprotein
• Reduction and/or loss of E-cadherin expression in carcinomas correlates positively with tumor cell invasion and metastasis
• Altered expression of E-cadherin is common in the progression of cancers including breast carcinoma, lung carcinoma, gastric tumors, hepatocellular carcinoma, and prostatic tumors
• It has recently been reported that E-cadherin is a useful marker for differentiating lobular lesions (negative) from ductal invasive lesions (positive) in breast cancer
• Positive tissue: Non-neural epithelia
• Reacts strongly with human E-cadherin and weakly with human P-cadherin
• Expected Staining Pattern: Mainly cellular membrane, some cytoplasmic E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily
• The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer
• Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis
• The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization
• Identified transcript variants arise from mutation at consensus splice sites
• E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity
• In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16
• Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.