200064044
CD33 Monoclonal Antibody (WM-53 (WM53)), Brilliant Violet™ 605, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD33
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Clone
WM-53 (WM53)
Applications
Flow Cytometry
Conjugate
Brilliant Violet 605
Host Species
Mouse
Target Species
Human
Gene Accession No.
P20138
Gene ID (Entrez)
945
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
CD33
Related Products
Description
- CD33 Monoclonal antibody specifically detects CD33 in Human samples
- It is validated for Flow Cytometry Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family
- It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells
- Applications Reported: This WM-53 (WM53) antibody has been reported for use in flow cytometric analysis
- Applications Tested: This WM-53 (WM53) antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Brilliant Violet™ 605 (BV605) is a tandem dye that emits at 605 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
- Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
- Our internal testing suggests that Brilliant Violet™ 605 (BV605) is not compatible with methanol-based fixation
- Excitation: 407 nm; Emission: 605 nm; Laser: Violet Laser
- BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
- Powered by Sirigen.™
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Antigen: CD33
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias: CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: WM-53 (WM53)
Applications: Flow Cytometry
Conjugate: Brilliant Violet 605
Host Species: Mouse
Target Species: Human
Gene Accession No.: P20138
Gene ID (Entrez): 945
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: CD33
Antigen:
CD33
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
WM-53 (WM53)
Applications:
Flow Cytometry
Conjugate:
Brilliant Violet 605
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P20138
Gene ID (Entrez):
945
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
CD33
Antigen: Desmoglein-3 (Squamous Cell Marker)
Concentration: 200 μg/mL
Classification: Recombinant Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias: 130 kDa pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen homolog; bal; balding; Cadherin family member 6; CDHF6; desmoglein 3; desmoglein-3; DKFZp686P23184; Dsg3; PVA
Isotype: IgG1 κ
Purification Method: Protein A/G
Product Type: Antibody
Clone: rDSG3/8611
Applications: Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P32926
Gene ID (Entrez): 1830
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Dsg3
Antigen:
Desmoglein-3 (Squamous Cell Marker)
Concentration:
200 μg/mL
Classification:
Recombinant Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias:
130 kDa pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen homolog; bal; balding; Cadherin family member 6; CDHF6; desmoglein 3; desmoglein-3; DKFZp686P23184; Dsg3; PVA
Isotype:
IgG1 κ
Purification Method:
Protein A/G
Product Type:
Antibody
Clone:
rDSG3/8611
Applications:
Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P32926
Gene ID (Entrez):
1830
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Dsg3
Antigen: CD107a/Lysosomal associated membrane protein 1 (LAMP1)
Concentration: 200 μg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias: 120 kDa lysosomal membrane glycoprotein; AI196048; CD107 antigen-like family member A; CD107a; I79_011073; Lamp I; LAMP1; LAMP-1; LAMPA; LGP120; LGP-120; LGPA; LGP-A; lysosomal associated membrane protein 1; Lysosomal associated membrane protein 1 (120 kDa); lysosomal membrane glycoprotein 1; lysosomal membrane glycoprotein A; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); lysosome-associated membrane protein 1; P2B
Isotype: IgG1 κ
Purification Method: Protein A/G
Product Type: Antibody
Clone: LAMP1/7456
Applications: Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P11279
Gene ID (Entrez): 3916
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: LAMP1
Antigen:
CD107a/Lysosomal associated membrane protein 1 (LAMP1)
Concentration:
200 μg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias:
120 kDa lysosomal membrane glycoprotein; AI196048; CD107 antigen-like family member A; CD107a; I79_011073; Lamp I; LAMP1; LAMP-1; LAMPA; LGP120; LGP-120; LGPA; LGP-A; lysosomal associated membrane protein 1; Lysosomal associated membrane protein 1 (120 kDa); lysosomal membrane glycoprotein 1; lysosomal membrane glycoprotein A; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); lysosome-associated membrane protein 1; P2B
Isotype:
IgG1 κ
Purification Method:
Protein A/G
Product Type:
Antibody
Clone:
LAMP1/7456
Applications:
Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P11279
Gene ID (Entrez):
3916
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
LAMP1
Antigen: CD57/B3GAT1 (Natural Killer Cell Marker)
Concentration: 200 μg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias: HNK-1; Leu-7
Isotype: IgG2 λ
Purification Method: Protein A/G
Product Type: Antibody
Clone: NK1/7566
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: Q9P2W7
Gene ID (Entrez): 27087
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: B3GAT1
Antigen:
CD57/B3GAT1 (Natural Killer Cell Marker)
Concentration:
200 μg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.5% BSA and 0.5% sodium azide
Gene Alias:
HNK-1; Leu-7
Isotype:
IgG2 λ
Purification Method:
Protein A/G
Product Type:
Antibody
Clone:
NK1/7566
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
Q9P2W7
Gene ID (Entrez):
27087
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
B3GAT1