Gata-3 Monoclonal Antibody (TWAJ), Brilliant Violet™ 605, eBioscience™, Invitrogen™

Description

• Gata-3 Monoclonal antibody specifically detects Gata-3 in Human, Mouse, Porcine, Rhesus Monkey samples
• It is validated for Flow Cytometry Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors
• Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation
• Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney
• During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal
• The Gata-3 is also essential for T cell commitment and survival
• In the thymus, expression is found mainly on the CD4 single positive cells
• During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation
• Alternative splice variants have been reported especially in the MCF7 cell line
• The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein
• Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00)
• Crossreactivity in rhesus monkeys has been published
• Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This TWAJ antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol
• Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins' located at <a href='https://www.thermofisher.com/flowprotocols'>Flow Protocols</a>
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Brilliant Violet™ 605 (BV605) is a tandem dye that emits at 605 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
• Our internal testing suggests that Brilliant Violet™ 605 (BV605) is not compatible with methanol-based fixation
• Excitation: 407 nm; Emission: 605 nm; Laser: Violet Laser
• BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
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