200064830

IL-22 Monoclonal Antibody (22URTI), Brilliant Ultra Violet™ 563, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

IL-22

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias

Cytokine Zcyto18; EGK_03903; il 22; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-21; IL22; IL-22; Il22a; IL-22a; IL-D110; ILN; ILTIF; IL-TIF; ILTIF alpha; IL-TIF alpha; ILTIFa; Interleukin; interleukin 10-related T cell-derived inducible factor; interleukin 22; Interleukin22; interleukin-22; Interleukin-22a; RGD1561292; TIFa; TIFIL-23; UNQ3099/PRO10096; ZCYTO18

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

Clone

22URTI

Applications

Flow Cytometry

Conjugate

Brilliant Ultraviolet 563

Host Species

Mouse

Target Species

Human

Gene Accession No.

Q9GZX6

Gene ID (Entrez)

50616

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

IL22

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Description

  • IL-22 Monoclonal antibody specifically detects IL-22 in Human samples
  • It is validated for Flow Cytometry Description: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22
  • IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells
  • Nevertheless, other T cells have also been shown to produce IL-22
  • In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo
  • A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22
  • Applications Reported: This 22URTI antibody has been reported for use in intracellular staining followed by flow cytometric analysis
  • Applications Tested: This 22URTI antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (<a href='https://www.thermofisher.com/order/catalog/product/88-8824-00'>Product # 88-8824-00</a>) and protocol
  • Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins' located at <a href='https://www.thermofisher.com/flowprotocols'>Flow Protocols</a>
  • This may be used at 5 μL (0.25 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye that emits at 564 nm and is intended for use on cytometers equipped with an μgtraviolet (355 nm) laser
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (<a href='https://www.thermofisher.com/order/catalog/product/SB-4401-42'>Product # SB-4401-42</a>) or Brilliant Stain Buffer™ (<a href='https://www.thermofisher.com/order/catalog/product/00-4409-75'>Product # 00-4409-75</a>) to minimize any non-specific polymer interactions
  • Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
  • Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (<a href='https://www.thermofisher.com/order/catalog/product/00-8222-49'>Product # 00-8222-49</a>) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (<a href='https://www.thermofisher.com/order/catalog/product/00-5333-54'>Product # 00-5333-54</a>) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
  • Excitation: 350 nm; Emission: 564 nm; Laser: Ultraviolet Laser
  • BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
  • Powered by Sirigen™.

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Antigen:
IL-22

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
Cytokine Zcyto18; EGK_03903; il 22; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-21; IL22; IL-22; Il22a; IL-22a; IL-D110; ILN; ILTIF; IL-TIF; ILTIF alpha; IL-TIF alpha; ILTIFa; Interleukin; interleukin 10-related T cell-derived inducible factor; interleukin 22; Interleukin22; interleukin-22; Interleukin-22a; RGD1561292; TIFa; TIFIL-23; UNQ3099/PRO10096; ZCYTO18

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
22URTI

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 563

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
Q9GZX6

Gene ID (Entrez):
50616

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
IL22

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Antigen:
PLK1

Concentration:
200 μg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.5% BSA and 0.5% sodium azide

Gene Alias:
cell cycle regulated protein kinase; Plk; PLK 1; Plk1; PLK-1; plk1.S; plx1; polo (Drosophia)-like kinase; polo like kinase; polo like kinase 1; polo like kinase 1 S homeolog; polo-like kinase; polo-like kinase 1; polo-like kinase homolog; polo-like protein kinase; serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14; Unknown (protein for MGC:133927); XELAEV_18047692mg

Isotype:
IgG1 κ

Purification Method:
Protein A/G

Product Type:
Antibody

Clone:
PLK1/7122

Applications:
ELISA, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P53350

Gene ID (Entrez):
5347

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
Plk1

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Antigen:
PLK1

Concentration:
200 μg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.5% BSA and 0.5% sodium azide

Gene Alias:
cell cycle regulated protein kinase; Plk; PLK 1; Plk1; PLK-1; plk1.S; plx1; polo (Drosophia)-like kinase; polo like kinase; polo like kinase 1; polo like kinase 1 S homeolog; polo-like kinase; polo-like kinase 1; polo-like kinase homolog; polo-like protein kinase; serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14; Unknown (protein for MGC:133927); XELAEV_18047692mg

Isotype:
IgG1 κ

Purification Method:
Protein A/G

Product Type:
Antibody

Clone:
PLK1/7122

Applications:
ELISA, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P53350

Gene ID (Entrez):
5347

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
Plk1

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Antigen:
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Concentration:
200 μg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
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Formulation:
PBS with 0.5% BSA and 0.5% sodium azide

Gene Alias:
Gm1206; Zfp488; Zinc finger protein 488; Znf488

Isotype:
IgG2a

Purification Method:
Protein A/G

Product Type:
Antibody

Clone:
PCRP-ZNF488-2D8

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
Q96MN9

Gene ID (Entrez):
118738

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
ZNF488