Ki-67 Recombinant Rat Monoclonal Antibody (SolA15), Alexa Fluor™ Plus 647, Invitrogen™

Description

• Ki-67 Recombinant Monoclonal antibody specifically detects Ki-67 in Human, Mouse samples
• It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin) Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data
• Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology
• Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments
• These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro
• Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment
• This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining
• Applications Tested: This SolA15 antibody has been tested by immunohistochemistry of mouse spleen tissue and immunocytochemistry and flow cytometric analysis of HeLa cells
• This may be used for immunocytochemistry at 0.5 μg/mL and for flow cytometry at less than or equal to 0.02-0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser Filtration: 0.2 μm post-manufacturing filtered.