CD197 (CCR7) Monoclonal Antibody (3D12), NovaFluor™ Yellow 700, eBioscience™, Invitrogen™

Description

• CD197 (CCR7) Monoclonal antibody specifically detects CD197 (CCR7) in Human samples
• It is validated for Flow Cytometry Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197
• CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21
• CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane
• CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells
• CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue
• Expression of CCR7 is also found on DC's
• During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation
• Expression of CCR7 on the cell surface can be down regulated upon ligand binding
• Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.6 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining
• Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer
• NovaFluor dyes are not compatible with DNA intercalating viability dyes
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI
• Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
• This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat
• No
• (<a href='https://www.thermofisher.com/order/catalog/product/C001T06F01'>C001T06F01</a>))
• This buffer contains formulation improvements over CellBlox
• CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used
• Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells
• Add 5 μL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended
• For single-color controls, use 5 μL of CellBlox Blocking Buffer per 100 μL of cell sample containing 10^3 to 10^8 cells
• NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts
• <a href = https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/novafluor-dyes.html>Learn more </a> Excitation: 552 nm; Emission: 700 nm; Laser: 561 nm (Yellow) Laser