Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Common beta Chain
1 mg/mL
Monoclonal
Purified
RUO
PBS
1439
IgG1 κ
Protein A purified
BION-1
Western Blot, Immunohistochemistry, Immunoprecipitation, Functional Assay, Neutralization
Unconjugated
Mouse
Human
beta, low-affinity, CD131, CD131 antigen, CDw131, colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage), cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta subunit, GM-CSF/IL-3/IL-5 receptor common beta-chain, IL3RB, interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity)
BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
Primary
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.