Antigen:
Integrin beta 2/CD18
Classification:
Monoclonal
Immunogen:
Blood was obtained from 4 helathy donors and the mononuclear cells harvested and grown. A BALB/c mouse was injected with 10^6 monocytes three times at monthly intervals, and four times with 5x10^6 monocytes oon each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest.
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Applications:
Flow Cytometry, ELISA, Immunofluorescence, Immunoprecipitation, Radioimmune Assays (RIA)
Target Species:
Human, Macaque
Gene Alias:
959 beta subunit precursor)10, CD18 antigen, CD18beta 2, cell surface adhesion glycoprotein (LFA-1, CR3, integrin beta-2, integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit), integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), LAD, LCAMB, leukocyte cell adhesion molecule CD18, leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C, MF17, MFI7, P150
Purification Method:
Protein A purified