Human IFN-alpha Antibody, R&D Systems™

IFN-alpha

Monoclonal

Unconjugated

Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) with No Preservative

IFNA2

Human Leukocyte Interferon

50 μg

Primary

One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) “Convenient Assay for Interferons,” J. Virol . 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) “A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon,” in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) “Interferon Standards and General Abbreviations,” in Methods in Enzymology (S. Pestka, ed.),

After receipt, the product may be stored at -20degreesC for short-term use ( < 6 months). For long-term storage, we recommend storing the product at -70degreesC or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

IgG1

ELISA, Neutralization

MMHA-11

ELISA, Neutralization

IFNA, IFNA2, IFNA2b, IFNalpha, IFN-alphaA, INFA2, interferon alpha 2, LeIF D

Mouse

Protein A or G purified from hybridoma culture supernatant

RUO

3440

Human

Purified