25-108-842

VISTA Monoclonal Antibody (B7H5DS8), PE-Cyanine7, eBioscience™, Invitrogen™

Manufacturer: eBioscience

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Pack Size SKU Availability Price
Each of 1 25-108-842-Each-of-1 In Stock ₹ 37,133.04

25-108-842 - Each of 1

₹ 37,133.04

In Stock

Quantity

1

Base Price: ₹ 37,133.04

GST (18%): ₹ 6,683.947

Total Price: ₹ 43,816.987

Antigen

VISTA

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

Q9H7M9

Gene Symbols

VSIR

Immunogen

VISTA-Fc protein

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

Applications

Flow Cytometry

Clone

B7H5DS8

Conjugate

PE-Cyanine7

Gene

VSIR

Gene Alias

4632428N05Rik; B7H5; B7-H5; C10orf54; C1H10orf54; C28H10orf54; C4H10orf54; chromosome 10 open reading frame 54; DD1alpha; Death Domain1alpha; Dies1; differentiation of ESCs 1; GI24; PD-1H; PDCD1 homolog; PD-H1; platelet receptor Gi24; PP2135; RIKEN cDNA 4632428N05 gene; SISP1; sisp-1; Stress-induced secreted protein-1; UNQ730/PRO1412; V-domain Ig suppressor of T cell activation; VISTA; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; VSIR; V-type immunoglobulin domain-containing suppressor of T-cell activation

Host Species

Mouse

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

64115

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

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Description

  • Description: This B7H5DS8 monoclonal antibody recognizes human VISTA, also known as B7-H5 or PD-1H
  • This clone has been tested and shown not to cross-react with human B7-H4, B7-H6 and B7-H7
  • This B7H5DS8 antibody will work in flow cytometry only on live and not paraformaldehyde-fixed cells
  • Applications Reported: This B7H5DS8 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This B7H5DS8 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
  • This may be used at 5 μL (0.125 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
  • Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene
  • Although VISTA bears sequence homology to PD-L1 it has a different expression pattern
  • In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells
  • It is usually absent on B cells
  • VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy
  • The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

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Show Difference

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eBioscience

25-108-842

--


Antigen:
VISTA

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q9H7M9

Gene Symbols:
VSIR

Immunogen:
VISTA-Fc protein

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

Applications:
Flow Cytometry

Clone:
B7H5DS8

Conjugate:
PE-Cyanine7

Gene:
VSIR

Gene Alias:
4632428N05Rik; B7H5; B7-H5; C10orf54; C1H10orf54; C28H10orf54; C4H10orf54; chromosome 10 open reading frame 54; DD1alpha; Death Domain1alpha; Dies1; differentiation of ESCs 1; GI24; PD-1H; PDCD1 homolog; PD-H1; platelet receptor Gi24; PP2135; RIKEN cDNA 4632428N05 gene; SISP1; sisp-1; Stress-induced secreted protein-1; UNQ730/PRO1412; V-domain Ig suppressor of T cell activation; VISTA; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; VSIR; V-type immunoglobulin domain-containing suppressor of T-cell activation

Host Species:
Mouse

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
64115

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

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Tocris Bioscience™

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Antigen:
__

Classification:
__

Concentration:
__

Formulation:
__

Gene Accession No.:
__

Gene Symbols:
__

Immunogen:
__

Quantity:
10 mg

Primary or Secondary:
__

Target Species:
__

Product Type:
CGS 35066

Isotype:
__

Applications:
__

Clone:
__

Conjugate:
__

Gene:
__

Gene Alias:
__

Host Species:
__

Purification Method:
__

Regulatory Status:
__

Gene ID (Entrez):
__

Content And Storage:
__

Form:
__

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Fischer Scientific

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Antigen:
CD122

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P16297

Gene Symbols:
Il2rb

Immunogen:
__

Quantity:
25 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2b κ

Applications:
Flow Cytometry

Clone:
TM-b1 (TM-beta1)

Conjugate:
PE-Cyanine7

Gene:
Il2rb

Gene Alias:
CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL-15 receptor beta chain; IL15RB; IL15Rbeta; IL-15Rbeta; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; Il-2/15Rbeta; IL-2R subunit beta; IL2RB; IL-2RB; IL2RBC; Il-2Rbeta; interleukin 15 receptor, beta; interleukin 2 receptor subunit beta; interleukin 2 receptor, beta; interleukin 2 receptor, beta chain; Interleukin-15 receptor subunit beta; Interleukin-2 receptor subunit beta; p70; p70-75; p75

Host Species:
Rat

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
16185

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

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Invitrogen

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Antigen:
CD126

Classification:
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Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
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Gene Symbols:
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Immunogen:
__

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

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Isotype:
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Flow Cytometry

Clone:
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Conjugate:
PE-Cyanine7

Gene:
Il6ra

Gene Alias:
CD126; CD126 antigen; gp80; IL-6 Receptor; il-6 receptor alpha; IL-6 receptor alpha chain; IL-6 receptor subunit alpha; IL6Q; IL6R; IL-6R; IL-6R 1; IL-6R subunit alpha; IL6R1; IL-6R-1; Il6ra; IL-6RA; IL-6R-alpha; IL6RQ; ILN; Interleukin; interleukin 6 receptor; interleukin 6 receptor, alpha; interleukin-6 receptor subunit alpha; Membrane glycoprotein 80; MGC104991

Host Species:
Rat

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
16194

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid