CD200R3 Monoclonal Antibody (Ba13), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: This Ba13 monoclonal antibody recognizes mouse CD200R3, a surface receptor expressed by basophils and mast cells
• Applications Reported: This Ba13 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This Ba13 antibody has been tested by flow cytometric analysis of the MC/9 cell line
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser CD200R3 is a dimeric cell surface receptor also known as CD200RLb
• CD200R3 contains two Ig-like extracellular domains and as such is a member of the immunoglobulin superfamily of receptors
• The intracellular region of CD200R3 associates with the adaptor molecule DAP12, and possibly also DAP10, CD3 zeta and FcR gamma-chain, indicating that it is capable of transducing stimulatory signals
• In this respect CD200R3 bears similarity to activatory receptors CD200R2 and CD200R4
• In contrast, an inhibitory receptor CD200R (also known as CD200R1) relies on other adaptors
• CD200R3 is primarily expressed by basophils and mast cells, cells known to be involved in anti-parasitic immune responses and frequently also allergic reactions
• The surface expression of CD200R3 decreases when basophils are activated by ligating their CD16 and CD32
• Remarkably, most of this downregulation is observed within the first 30 minutes after activation
• Unlike CD200R, CD200R3 does not bind CD200.