Tmem119 Monoclonal Antibody (V3RT1GOsz), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: This V3RT1GOsz monoclonal antibody recognizes mouse Tmem119
• This clone has been reported to be successfully used in flow cytometry
• Applications Reported: This V3RT1GOsz antibody has been reported for use in flow cytometric analysis
• Applications Tested: This V3RT1GOsz antibody has been tested by flow cytometric analysis of mouse brain cells after myelin removal
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2
• Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages
• Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons
• They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment
• Microglia are particularly mobile in areas of the brain undergoing restructuring
• Reactive microglia co-express the marker Iba1
• In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis
• The specific function Tmem119 plays on microglial cells remains unclear
• Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.