IL-4 Monoclonal Antibody (11B11), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine
• The 11B11 antibody is a neutralizing antibody
• Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells
• IL-4 is species-specific and promotes proliferation and differentiation of B cells
• IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23
• Applications Reported: This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 11B11 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
• This antibody can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• IL-4 Monoclonal antibody specifically detects IL-4 in Mouse samples
• It is validated for Flow Cytometry.