IFN gamma Monoclonal Antibody (4S.B3), Brilliant Ultra Violet™ 395, eBioscience™, Invitrogen™

Description

• The 4S.B3 mAb reacts with interferon-gamma
• Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine
• IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells
• Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma
• Glycosylation of IFN gamma does not affect its biological activity
• AApplications Tested: This 4S.B3 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (88-8824-00) and protocol
• Please refer to Staining Intracellular Antigens for Flow Cytometry
• This may be used at 5uL (1ug) per test BUV395 is a dye that emits at 395nm and is intended for use on cytometers equipped with an Ultraviolet (355nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, BV, BUV, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Excitation: 350nm; Emission: 395nm; Laser: Ultraviolet Laser
• IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
• IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
• IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
• On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
• The biological activity of the IFN gamma homodimer is highly species specific
• Human IFN gamma does not show cross-reactivity with mouse
• IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
• IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
• Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
• IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
• IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
• IFN gamma gene mutations are associated with aplastic anemia.