CD4 Monoclonal Antibody (RM4-5), Brilliant Ultra Violet™ 496, eBioscience™, Invitrogen™

Description

• The RM4-5 mAb reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells
• CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells
• In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail
• Binding of RM4-5 is blocked by GK1.5
• Applications Reported: This RM4-5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RM4-5 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 1.0μg per test Brilliant Ultra Violet 496 (BUV496) is a tandem dye that emits at 496 nm and is intended for use on cytometers equipped with an Ultraviolet (355 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Excitation: 350 nm; Emission: 496 nm; Laser: Ultraviolet Laser
• The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
• CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
• At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
• CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
• It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
• Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].