CD19 Monoclonal Antibody (HIB19), Brilliant Ultra Violet™ 737, eBioscience™, Invitrogen™

Description

• CD19 Monoclonal antibody specifically detects CD19 in Human samples
• It is validated for Flow Cytometry Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein
• CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells
• Follicular dendritic cells also express CD19
• Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR
• Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells
• The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes
• Applications Reported: This HIB19 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HIB19 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an μgtraviolet (355 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
• Our internal testing suggests that Brilliant Ultra Violet™ 737 (BUV737) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour
• Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
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