CD39 Monoclonal Antibody (24DMS1), Brilliant Ultra Violet™ 805, eBioscience™, Invitrogen™

Description

• The 24DMS1 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI
• E-NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP
• CD39 is the dominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation
• CD39 can impact expression of CD73, another E-NTPase
• Together, these molecules influence inflammation responses
• CD39 is expressed on B cells, Langerhans cells and most monocytes
• In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T reg cells
• T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo
• Applications Reported: This 24DMS1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 24DMS1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Brilliant Ultraviolet 805 is a tandem dye that emits at 797 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser
• CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes
• CD39 has intrinsic ecto-ATPase activity
• Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.