Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Ultra Violet™ 805, eBioscience™, Invitrogen™

Description

• The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1)
• This A1exF5 clone is compatible with both, the standard intracellular protocols, and the Foxp3/Transcription Factor Staining Buffer Set
• Applications Reported: This A1exF5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This A1exF5 antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00)
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Brilliant Ultraviolet 805 is a tandem dye that emits at 797 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser
• Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle
• The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation
• By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production
• In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells
• In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported
• In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a)
• Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas
• Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.