p67 (phox) Mouse, Unlabeled, Clone: 29, BD

Description

• The neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli
• The active site of this enzyme is located in an integral membrane cytochrome, b558, that consists of the two subunits gp91 [phox] and p21 [phox]
• Superoxide production depends on the formation of a complex that includes p67 [phox], p47 [phox], and the GTP-binding protein Rac
• Upon activation, these proteins translocate from the cytosol to the membrane where they assemble with b558 and induce oxidase activity
• p67 [phox] contains two SH3 domains and binds, via its C-terminal SH3 domain, to the proline rich region of p47 [phox]
• This binding allows p67 [phox] to indirectly associate with the oxidase
• It is thought that the phosphorylated forms of p67 [phox] and p47 [phox] interact and that the phosphorylation of p67 [phox] is regulated by both PKC-dependent and independent pathways
• Although the role of p67 [phox] in electron flow control is poorly understood, it is thought that it regulates the transfer of electrons from NADPH to reduce flavin
• Immunofluorescence, Western Blotting