Dynamin II Mouse, Unlabeled, Clone: 27, BD

Description

• Dynamin is a membrane-associated GTPase that binds to GTP, microtubules, and phospholipids
• Northern blot analysis has shown dynamin to be present in most tissues but at an amount about 20-fold lower than that in brain
• There are at least two distinct dynamin genes in mammals
• Transcripts of both dynamin genes are alternatively spliced at two or more sites
• The first site is identical in bothdynamins, whereas, the second site differs
• The two gene products are known as Dynamin I and Dynamin II and show 79% identity
• Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous
• The two proteins are highly homologous in the N-terminal region, while the C-terminal domain shows significant divergence
• The GTPase activity of Dynamin I is stimulated several fold by binding to microtubules, phospholipids, and membranous vesicles
• Dynamin I is a good substrate of PKC in vitro as well as in vivo in resting nerve terminals
• In vitro, phosphorylation occurs at the C-terminus of Dynamin I and this enhances the GTPase activity more than 10-fold
• However, Dynamin II is not a substrate of PKC and its activity does not appear to be affected by phosphorylation
• These data suggest that the function of the common N-terminal domain between Dynamin I and II may be differentially specified by distinct C-terminal domains.