3855930

SNAP-25 Mouse, Unlabeled, Clone: 20, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

SNAP-25

Concentration

250μg/mL

Classification

Monoclonal

Host Species

Mouse

Research Discipline

Cell Biology

Formulation

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Isotype

IgG1

Purification Method

Affinity Purified

Description

Synaptosomal Associated Protein of 25 kD

Clone

20

Applications

Immunofluorescence, Western Blot

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Mouse, Rat

Immunogen

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Primary or Secondary

Primary

Content And Storage

Store undiluted at -20°C.

Description

  • Release of neurotransmitters from neurons is regulated by exocytosis of synaptic vesicles
  • This exocytosis is mediated by a complex consisting of membrane components of both the synaptic vesicle and the synaptic plasma membrane
  • The fusion complex consists of the soluble NSF (N-ethyl-maleimide-sensitive factor) and SNAPs (soluble NSF attachment proteins), along with the receptor proteins (known as SNAREs) synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosomal-associated protein of 25kDa- the name is coincidental to the previously mentioned “SNAP” terminology)
  • SNAP-25 and syntaxin are plasmalemmal proteins (designated as t-SNAREs) while synaptobrevin and synaptotagmin are vesicular proteins (designated as v-SNAREs)
  • These four proteins are thought to constitute an initial SNARE docking complex for regulated exocytosis
  • SNAP-25 lacks a transmembrane domain, but is linked to the membrane by palmitoylated cysteine residues in the central region of the molecule
  • Immunofluorescence, Immunoprecipitation, Western Blotting

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Antigen:
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Concentration:
250μg/mL

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Research Discipline:
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Formulation:
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Isotype:
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Purification Method:
Affinity Purified

Description:
Synaptosomal Associated Protein of 25 kD

Clone:
20

Applications:
Immunofluorescence, Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
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Isotype:
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Conjugate:
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Mouse Adaptin γ aa. 642-821

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Store undiluted at -20°C.

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Research Discipline:
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Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

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