3878638

CD3 Mouse anti-Human, V500, Clone: SP34-2, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD3

Applications

Flow Cytometry

Conjugate

Violet Fluor 500 (V500)

Regulatory Status

RUO

Target Species

Baboon, Cynomolgus, Rhesus Monkey

Immunogen

Purified Human CD3η Protein

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone

SP34-2

Classification

Monoclonal

Host Species

Mouse

Research Discipline

Immunology

Formulation

Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Isotype

IgG1 λ

Purification Method

Affinity Purified

Description

  • Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone
  • It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys
  • The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cells markers
  • SP34-2 is also capable of inducing cell proliferation on both human and non-human primate PBMC
  • The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
  • It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500nm
  • BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel
  • For more information on BD Horizon V500, visit bdbiosciences.com/colors
  • When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls
  • Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents
  • Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
  • Different V500 reagents (e.g
  • CD4 vs
  • CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents
  • Flow Cytometry

Compare Similar Items

Show Difference

Img

Fischer Scientific

3878638

Mouse Monoclonal Antibody...


Antigen:
CD3

Applications:
Flow Cytometry

Conjugate:
Violet Fluor 500 (V500)

Regulatory Status:
RUO

Target Species:
Baboon, Cynomolgus, Rhesus Monkey

Immunogen:
Purified Human CD3η Protein

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
SP34-2

Classification:
Monoclonal

Host Species:
Mouse

Research Discipline:
Immunology

Formulation:
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Isotype:
IgG1 λ

Purification Method:
Affinity Purified

Img

Fischer Scientific

3878792

Mouse Monoclonal Antibody...


Antigen:
CD107a

Applications:
Flow Cytometry

Conjugate:
APC

Regulatory Status:
RUO

Target Species:
Human

Immunogen:
Human Adult Adherent Peripheral Blood Cells

Primary or Secondary:
Primary

Content And Storage:
__

Clone:
H4A3

Classification:
Monoclonal

Host Species:
Mouse

Research Discipline:
__

Formulation:
__

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Img

Fischer Scientific

3878933

Mouse Monoclonal Antibody...


Antigen:
CD4

Applications:
Flow Cytometry

Conjugate:
Violet Fluor 500 (V500)

Regulatory Status:
RUO

Target Species:
Human

Immunogen:
__

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
RPA-T4

Classification:
Monoclonal

Host Species:
Mouse

Research Discipline:
Immunology

Formulation:
Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Img

Fischer Scientific

3878939

Rat Monoclonal Antibody...


Antigen:
CD8a

Applications:
Flow Cytometry

Conjugate:
Violet Fluor 500 (V500)

Regulatory Status:
RUO

Target Species:
Mouse

Immunogen:
Mouse thymus / spleen Cells

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
53-6.7

Classification:
Monoclonal

Host Species:
Rat

Research Discipline:
Immunology

Formulation:
Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.

Isotype:
IgG2a κ

Purification Method:
Affinity Purified