GGA3 Mouse anti-Human, Unlabeled, Clone: 8, BD

Description

• The ADP-ribosylation factors (ARFs) are a family of small GTPases in the ARF superfamily that include ARFs and ARF-like (ARLs) proteins
• At least six ARFs have been identified in humans: ARF1, ARF2, ARF3, ARF4, ARF5, and ARF6
• ARFs are involved in intravesicular acidification and fusion of microsomal vesicles, endosome fusion, nuclear membrane assembly, and formation of clathrin-coated vesicles
• GGAs are ARF-binding proteins that act as adaptor coat proteins associated with the Golgi complex
• GGA1, GGA2, and GGA3 are homologous proteins that contain N-terminal VHS domains, a GGA and TOM homology region (GAT), and a C-terminal region homologous to the ear domain of γ-adaptins
• GGAs co-localize with Golgi markers in the TGN, and GGA3 is found present in coated vesicles and buds associated with the TGN
• The GAT domain of GGA3 facilitates ARF1 binding, Golgi localization, and dissociation from ARF-regulated membranes
• The C-terminal region of GGAs bind to MAP1A and rabaptin-5, which are binding partners of γ-adaptins
• Overexpression of GGAs alters the distribution of markers normally found in the TGN
• Thus, GGAs are ARF binding proteins that regulate vesicle dynamics in the TGN
• Immunofluorescence, Western Blotting