3969576

CD4 Mouse anti-Human, V450, Clone: RPA-T4, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD4

Applications

Flow Cytometry

Conjugate

Violet Fluor 450 (V450)

Regulatory Status

RUO

Target Species

Human

Isotype

IgG1 κ

Purification Method

Affinity Purified

Clone

RPA-T4

Classification

Monoclonal

Host Species

Mouse

Research Discipline

Immunology

Formulation

Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Description

  • The RPA-T4 clone reacts with CD4, a 59kDa single-chain transmembrane glycoprotein (receptor for human immunodeficiency virus (HIV)) present on T-helper/inducer cell populations
  • This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and reacts with approximately 80% of thymocytes and 45% of peripheral blood lymphocytes
  • CD4 is also present in low density on peripheral blood monocytes
  • RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation
  • The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
  • It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm
  • Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates
  • Host Species: Mouse Clone: RPA-T4 Isotype: IgG1 κ Species Reactivity: Human Flow Cytometry, Functional Assay

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