40-699-6642
Gata-3 Monoclonal Antibody (TWAJ), Brilliant Violet™ 605, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 40-699-6642-Each-of-1 | In Stock | ₹ 32,427.24 |
40-699-6642 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 32,427.24
GST (18%): ₹ 5,836.903
Total Price: ₹ 38,264.143
Antigen
Gata-3
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P23771, P23772
Host Species
Rat
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human, Mouse, Porcine, Rhesus Monkey
Product Type
Antibody
Isotype
IgG2b κ
Applications
Flow Cytometry
Clone
TWAJ
Conjugate
Brilliant Violet 605
Gene
GATA3
Gene Alias
EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
14462, 2625, 713840, 733631
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
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Description
- Gata-3 Monoclonal antibody specifically detects Gata-3 in Human, Mouse, Porcine, Rhesus Monkey samples
- It is validated for Flow Cytometry Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors
- Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation
- Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney
- During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal
- The Gata-3 is also essential for T cell commitment and survival
- In the thymus, expression is found mainly on the CD4 single positive cells
- During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation
- Alternative splice variants have been reported especially in the MCF7 cell line
- The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein
- Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00)
- Crossreactivity in rhesus monkeys has been published
- Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This TWAJ antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol
- Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins' located at <a href='https://www.thermofisher.com/flowprotocols'>Flow Protocols</a>
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Brilliant Violet™ 605 (BV605) is a tandem dye that emits at 605 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
- Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
- Our internal testing suggests that Brilliant Violet™ 605 (BV605) is not compatible with methanol-based fixation
- Excitation: 407 nm; Emission: 605 nm; Laser: Violet Laser
- BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
- Powered by Sirigen.™
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Show Difference
Antigen: Gata-3
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.: P23771, P23772
Host Species: Rat
Quantity: 100 Tests
Primary or Secondary: Primary
Target Species: Human, Mouse, Porcine, Rhesus Monkey
Product Type: Antibody
Isotype: IgG2b κ
Applications: Flow Cytometry
Clone: TWAJ
Conjugate: Brilliant Violet 605
Gene: GATA3
Gene Alias: EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 14462, 2625, 713840, 733631
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Antigen:
Gata-3
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.:
P23771, P23772
Host Species:
Rat
Quantity:
100 Tests
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Porcine, Rhesus Monkey
Product Type:
Antibody
Isotype:
IgG2b κ
Applications:
Flow Cytometry
Clone:
TWAJ
Conjugate:
Brilliant Violet 605
Gene:
GATA3
Gene Alias:
EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
14462, 2625, 713840, 733631
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Antigen: IgM
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.: P01871
Host Species: Mouse
Quantity: 25 Tests
Primary or Secondary: Primary
Target Species: Human
Product Type: __
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: SA-DA4
Conjugate: Brilliant Violet 605
Gene: IGHM
Gene Alias: AGM1, IGHM, immunoglobulin heavy constant mu, MU, VH
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 3507
Content And Storage: 4°, C, store in dark, DO NOT FREEZE!
Form: Liquid
Antigen:
IgM
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.:
P01871
Host Species:
Mouse
Quantity:
25 Tests
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
__
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
SA-DA4
Conjugate:
Brilliant Violet 605
Gene:
IGHM
Gene Alias:
AGM1, IGHM, immunoglobulin heavy constant mu, MU, VH
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
3507
Content And Storage:
4°, C, store in dark, DO NOT FREEZE!
Form:
Liquid
Antigen: IgM
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.: P01871
Host Species: Mouse
Quantity: 100 Tests
Primary or Secondary: Primary
Target Species: Human
Product Type: __
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: SA-DA4
Conjugate: Brilliant Violet 605
Gene: IGHM
Gene Alias: AGM1, IGHM, immunoglobulin heavy constant mu, MU, VH
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 3507
Content And Storage: 4°, C, store in dark, DO NOT FREEZE!
Form: Liquid
Antigen:
IgM
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.:
P01871
Host Species:
Mouse
Quantity:
100 Tests
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
__
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
SA-DA4
Conjugate:
Brilliant Violet 605
Gene:
IGHM
Gene Alias:
AGM1, IGHM, immunoglobulin heavy constant mu, MU, VH
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
3507
Content And Storage:
4°, C, store in dark, DO NOT FREEZE!
Form:
Liquid
Antigen: CD3e
Classification: Monoclonal
Concentration: 0.2 mg/mL
Formulation: PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.: P04235, P11942, P22646, P24161
Host Species: Armenian Hamster
Quantity: 25 μg
Primary or Secondary: Primary
Target Species: Mouse
Product Type: __
Isotype: IgG
Applications: Flow Cytometry
Clone: 145-2C11
Conjugate: Brilliant Violet 711
Gene: CD3g
Gene Alias: 4930549J05Rik, A430104F18Rik, AI504783, antigen CD3D, delta polypeptide (TiT3 complex), antigen CD3E, epsilon polypeptide (TiT3 complex), antigen CD3G, gamma polypeptide, antigen CD3Z, zeta polypeptide, AW552088, Cd247, CD247 antigen, CD247 antigen, zeta subunit, Cd247 molecule, CD3, CD3 antigen delta chain, CD3 antigen delta polypeptide, CD3 antigen gamma chain, CD3 antigen, delta polypeptide, CD3 antigen, delta subunit, CD3 antigen, epsilon polypeptide, CD3 antigen, gamma polypeptide, CD3 antigen, zeta polypeptide, CD3 delta, CD3 epsilon, CD3 epsilon chain, CD3 epsilon subunit, CD3 epsilon subunit precursor, CD3 gamma-chain, CD3 glycoprotein, CD3 glycoprotein precursor, CD3 molecule delta polypeptide, CD3 molecule, delta, CD3 molecule, epsilon, CD3 molecule, epsilon polypeptide, CD3 molecule, gamma, CD3 molecule, gamma polypeptide, CD3 protein, CD3 TCR complex, CD3 type I transmembrane glycoprotein, CD3 type I transmembrane glycoprotein precursor, CD3 zeta chain, Cd3d, CD3D antigen delta, CD3D antigen, delta polypeptide (TiT3 complex), CD3d molecule, CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, Cd3e, CD3E antigen, epsilon polypeptide, CD3E antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, CD3e molecule, epsilon (CD3-TCR complex), CD3epsilon, CD3-epsilon, Cd3-eta, Cd3g, CD3G antigen, gamma polypeptide, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, Cd3h, CD3Q, Cd3z, CD3Z antigen, zeta polypeptide (TiT3 complex), Cd3zeta, Cd3-zeta, CD3zeta chain, CD3-zeta/eta, Ctg3, Ctg-3, FLJ18683, IMD17, IMD18, IMD19, IMD25, Leu-4, OKT3, delta chain, T cell antigen receptor complex epsilon subunit of T3, T3/TCR complex, T3d, T3e, T3g, T3Z, T-cell antigen receptor complex, epsilon subunit of T3, T-cell antigen receptor complex, gamma subunit of T3, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor CD3 epsilon chain, T-cell receptor CD3 epsilon subunit, T-cell receptor CD3 subunit zeta, T-cell receptor CD3, subunit zeta, T-cell receptor T3 delta chain, T-cell receptor T3 eta chain, T-cell receptor T3 gamma chain, T-cell receptor T3 zeta chain, T-cell receptor zeta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface glycoprotein CD3 gamma chain, T-cell surface glycoprotein CD3 zeta chain, T-cell surface protein, TcR CD3 delta-chain, TcR CD3 gamma-chain, TCR zeta chain, TCR zeta chain subunit, TCRE, Tcrk, TCRZ, TCRzeta, TiT3 complex, type I transmembrane protein, T-cell surface molecule CD3
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 12500, 12501, 12502, 12503
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Antigen:
CD3e
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Formulation:
PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.:
P04235, P11942, P22646, P24161
Host Species:
Armenian Hamster
Quantity:
25 μg
Primary or Secondary:
Primary
Target Species:
Mouse
Product Type:
__
Isotype:
IgG
Applications:
Flow Cytometry
Clone:
145-2C11
Conjugate:
Brilliant Violet 711
Gene:
CD3g
Gene Alias:
4930549J05Rik, A430104F18Rik, AI504783, antigen CD3D, delta polypeptide (TiT3 complex), antigen CD3E, epsilon polypeptide (TiT3 complex), antigen CD3G, gamma polypeptide, antigen CD3Z, zeta polypeptide, AW552088, Cd247, CD247 antigen, CD247 antigen, zeta subunit, Cd247 molecule, CD3, CD3 antigen delta chain, CD3 antigen delta polypeptide, CD3 antigen gamma chain, CD3 antigen, delta polypeptide, CD3 antigen, delta subunit, CD3 antigen, epsilon polypeptide, CD3 antigen, gamma polypeptide, CD3 antigen, zeta polypeptide, CD3 delta, CD3 epsilon, CD3 epsilon chain, CD3 epsilon subunit, CD3 epsilon subunit precursor, CD3 gamma-chain, CD3 glycoprotein, CD3 glycoprotein precursor, CD3 molecule delta polypeptide, CD3 molecule, delta, CD3 molecule, epsilon, CD3 molecule, epsilon polypeptide, CD3 molecule, gamma, CD3 molecule, gamma polypeptide, CD3 protein, CD3 TCR complex, CD3 type I transmembrane glycoprotein, CD3 type I transmembrane glycoprotein precursor, CD3 zeta chain, Cd3d, CD3D antigen delta, CD3D antigen, delta polypeptide (TiT3 complex), CD3d molecule, CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, Cd3e, CD3E antigen, epsilon polypeptide, CD3E antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, CD3e molecule, epsilon (CD3-TCR complex), CD3epsilon, CD3-epsilon, Cd3-eta, Cd3g, CD3G antigen, gamma polypeptide, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, Cd3h, CD3Q, Cd3z, CD3Z antigen, zeta polypeptide (TiT3 complex), Cd3zeta, Cd3-zeta, CD3zeta chain, CD3-zeta/eta, Ctg3, Ctg-3, FLJ18683, IMD17, IMD18, IMD19, IMD25, Leu-4, OKT3, delta chain, T cell antigen receptor complex epsilon subunit of T3, T3/TCR complex, T3d, T3e, T3g, T3Z, T-cell antigen receptor complex, epsilon subunit of T3, T-cell antigen receptor complex, gamma subunit of T3, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor CD3 epsilon chain, T-cell receptor CD3 epsilon subunit, T-cell receptor CD3 subunit zeta, T-cell receptor CD3, subunit zeta, T-cell receptor T3 delta chain, T-cell receptor T3 eta chain, T-cell receptor T3 gamma chain, T-cell receptor T3 zeta chain, T-cell receptor zeta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface glycoprotein CD3 gamma chain, T-cell surface glycoprotein CD3 zeta chain, T-cell surface protein, TcR CD3 delta-chain, TcR CD3 gamma-chain, TCR zeta chain, TCR zeta chain subunit, TCRE, Tcrk, TCRZ, TCRzeta, TiT3 complex, type I transmembrane protein, T-cell surface molecule CD3
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
12500, 12501, 12502, 12503
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid