41-771-7941
IL-17A Monoclonal Antibody (eBio64DEC17), Brilliant Violet™ 786, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 41-771-7941-Each-of-1 | In Stock | ₹ 15,315.24 |
41-771-7941 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 15,315.24
GST (18%): ₹ 2,756.743
Total Price: ₹ 18,071.983
Antigen
IL-17A
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
Q16552
Host Species
Mouse
Quantity
25 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Applications
Flow Cytometry
Clone
eBio64DEC17
Conjugate
Brilliant Violet 786
Gene
IL17A
Gene Alias
Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3605
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Related Products
Description
- IL-17A Monoclonal antibody specifically detects IL-17A in Human samples
- It is validated for Flow Cytometry Description: The eBio64DEC17 antibody reacts with human IL-17A
- The eBio64DEC17 antibody is a neutralizing antibody
- Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection
- The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8)
- Human IL-17A exists as glycosylated 20-30 kD homodimers
- High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells
- IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts
- Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2
- In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils
- Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor
- IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways
- The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells
- Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies
- Applications Reported: This eBio64DEC17 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This eBio64DEC17 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol
- Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins' located at <a href='https://www.thermofisher.com/flowprotocols'>Flow Protocols</a>
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Brilliant Violet™ 786 (BV786) is a tandem dye that emits at 786 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
- Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically
- Our internal testing suggests that Brilliant Violet™ 786 (BV786) is not compatible with methanol-based fixation
- Excitation: 407 nm; Emission: 786 nm; Laser: Violet Laser
- BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
- Powered by Sirigen.™
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Show Difference
Antigen: IL-17A
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.: Q16552
Host Species: Mouse
Quantity: 25 Tests
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: eBio64DEC17
Conjugate: Brilliant Violet 786
Gene: IL17A
Gene Alias: Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 3605
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Antigen:
IL-17A
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.:
Q16552
Host Species:
Mouse
Quantity:
25 Tests
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
eBio64DEC17
Conjugate:
Brilliant Violet 786
Gene:
IL17A
Gene Alias:
Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
3605
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Antigen: IL-17A
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.: Q16552
Host Species: Mouse
Quantity: 100 Tests
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: eBio64DEC17
Conjugate: Brilliant Violet 786
Gene: IL17A
Gene Alias: Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 3605
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Antigen:
IL-17A
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.:
Q16552
Host Species:
Mouse
Quantity:
100 Tests
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
eBio64DEC17
Conjugate:
Brilliant Violet 786
Gene:
IL17A
Gene Alias:
Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
3605
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Antigen: TNF alpha
Classification: Monoclonal
Concentration: 0.2 mg/mL
Formulation: PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.: P06804
Host Species: Rat
Quantity: 25 μg
Primary or Secondary: Primary
Target Species: Mouse
Product Type: __
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: MP6-XT22
Conjugate: Brilliant Violet 786
Gene: Tnf
Gene Alias: APC1 protein, Cachectin, C-domain 1, C-domain 2, cTNF, DADB-70P7.1, DIF, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, RATTNF, Tnf, TNF alpha, TNF superfamily, TNF a, TNF, macrophage-derived, TNF, monocyte-derived, TNFA, TNF-a, TNFalpha, TNF-alpha, Tnfsf1a, TNFSF2, TNFa, TNLG1F, Tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor alpha (cachetin), tumor necrosis factor alpha precursor, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form, tumor necrosis factor-alpha, tumor necrosis factor-alpha precursor, tumor-necrosis factor, tumour necrosis factor
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 21926
Content And Storage: 4°, C, store in dark, DO NOT FREEZE!
Form: Liquid
Antigen:
TNF alpha
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Formulation:
PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.:
P06804
Host Species:
Rat
Quantity:
25 μg
Primary or Secondary:
Primary
Target Species:
Mouse
Product Type:
__
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
MP6-XT22
Conjugate:
Brilliant Violet 786
Gene:
Tnf
Gene Alias:
APC1 protein, Cachectin, C-domain 1, C-domain 2, cTNF, DADB-70P7.1, DIF, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, RATTNF, Tnf, TNF alpha, TNF superfamily, TNF a, TNF, macrophage-derived, TNF, monocyte-derived, TNFA, TNF-a, TNFalpha, TNF-alpha, Tnfsf1a, TNFSF2, TNFa, TNLG1F, Tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor alpha (cachetin), tumor necrosis factor alpha precursor, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form, tumor necrosis factor-alpha, tumor necrosis factor-alpha precursor, tumor-necrosis factor, tumour necrosis factor
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
21926
Content And Storage:
4°, C, store in dark, DO NOT FREEZE!
Form:
Liquid
Antigen: TNF alpha
Classification: Monoclonal
Concentration: 0.2 mg/mL
Formulation: PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.: P06804
Host Species: Rat
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Mouse
Product Type: __
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: MP6-XT22
Conjugate: Brilliant Violet 786
Gene: Tnf
Gene Alias: APC1 protein, Cachectin, C-domain 1, C-domain 2, cTNF, DADB-70P7.1, DIF, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, RATTNF, Tnf, TNF alpha, TNF superfamily, TNF a, TNF, macrophage-derived, TNF, monocyte-derived, TNFA, TNF-a, TNFalpha, TNF-alpha, Tnfsf1a, TNFSF2, TNFa, TNLG1F, Tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor alpha (cachetin), tumor necrosis factor alpha precursor, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form, tumor necrosis factor-alpha, tumor necrosis factor-alpha precursor, tumor-necrosis factor, tumour necrosis factor
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 21926
Content And Storage: 4°, C, store in dark, DO NOT FREEZE!
Form: Liquid
Antigen:
TNF alpha
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Formulation:
PBS with BSA and 0.09% sodium azide, pH 7.2
Gene Accession No.:
P06804
Host Species:
Rat
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Mouse
Product Type:
__
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
MP6-XT22
Conjugate:
Brilliant Violet 786
Gene:
Tnf
Gene Alias:
APC1 protein, Cachectin, C-domain 1, C-domain 2, cTNF, DADB-70P7.1, DIF, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, RATTNF, Tnf, TNF alpha, TNF superfamily, TNF a, TNF, macrophage-derived, TNF, monocyte-derived, TNFA, TNF-a, TNFalpha, TNF-alpha, Tnfsf1a, TNFSF2, TNFa, TNLG1F, Tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor alpha (cachetin), tumor necrosis factor alpha precursor, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form, tumor necrosis factor-alpha, tumor necrosis factor-alpha precursor, tumor-necrosis factor, tumour necrosis factor
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
21926
Content And Storage:
4°, C, store in dark, DO NOT FREEZE!
Form:
Liquid