4697134

CD14 Mouse anti-Human, Brilliant Violet 650, Clone: M5E2, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD14

Applications

Flow Cytometry

Conjugate

Brilliant Violet 650

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype

IgG2a κ

Purification Method

Affinity Purified

Description

LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein

Clone

M5E2

Classification

Monoclonal

Host Species

Mouse

Target Species

Baboon, Canine, Cynomolgus, Human, Rhesus Monkey

Immunogen

Human CD14 Protein

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Description

  • The M5E2 monoclonal antibody specifically binds to CD14, a 53–55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes
  • Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells
  • CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB
  • The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
  • This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
  • BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
  • Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
  • However, the spillover can be corrected through compensation as with any other dye combination.

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Img

Fischer Scientific

4697134

Mouse Monoclonal Antibody...


Antigen:
CD14

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 650

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Description:
LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein

Clone:
M5E2

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Baboon, Canine, Cynomolgus, Human, Rhesus Monkey

Immunogen:
Human CD14 Protein

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

Fischer Scientific

4697137

Mouse Monoclonal Antibody...


Antigen:
CD14

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 421

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG2b κ

Purification Method:
Affinity Purified

Description:
LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein

Clone:
MPHIP9

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
Human Monocytes

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

Fischer Scientific

4697137

Mouse Monoclonal Antibody...


Antigen:
CD14

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 421

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG2b κ

Purification Method:
Affinity Purified

Description:
LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein

Clone:
MPHIP9

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
Human Monocytes

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

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4697153

Mouse Monoclonal Antibody...


Antigen:
CD45RO

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 711

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Description:
CD45R; PTPRC; LCA; Leukocyte common antigen; GP180; LY5; T200

Clone:
UCHL1

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
Human IL-2-dependent T-cell line

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.