CD16 Mouse anti-Human, Brilliant Violet 786, Clone: 3G8, BD

Description

• The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (Fc γRIII), a human NK cell-associated antigen
• CD16 is expressed on NK cells as well as macrophages and granulocytes
• Reports indicate that CD16 plays a role in signal transduction and NK cell activation
• The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes
• The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both Fc γRIIa and Fc γRIIIb molecules
• This antibody has also been reported to induce homotypic neutrophil aggregation
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm
• BD Horizon™ BV786 can be excited by the violet laser and detected in a filter used to detect Cy7™-like dyes (eg, 780/60-nm filter).