Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Target Species:
Human, Mouse
Gene Alias:
EC 1.14.13.39, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, INOS, nitric oxide synthase 2, inducible, nitric oxide synthase 2A (inducible, hepatocytes), nitric oxide synthase, inducible, nitric oxide synthase, macrophage, NOS, NOS type II, NOS2A, Peptidyl-cysteine S-nitrosylase NOS2
Immunogen:
iNOS Antibody (4E5) was made to a purified recombinant fragment of human iNOS (C-terminus) expressed in E. coli. [UniProt# P35228]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Dilution:
Western Blot 1:500-1:2000, Flow Cytometry 1:200-1:400, ELISA 1:10000, Immunohistochemistry reported in scientific literature (PMID 29891729), Immunohistochemistry-Paraffin 1:200-1:1000, Immunohistochemistry-Frozen reported in scientific literature (PMID 32780728), Flow (Intracellular)
Classification:
Monoclonal
Gene Accession No.:
P35228
Purification Method:
Ammonium sulfate precipitation