p73 Antibody (5B429) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 56 publications
Manufacturer: Fischer Scientific
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Antigen
p73
Concentration
1.0 mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Apoptosis, Cancer, Tumor Suppressors
Gene Accession No.
O15350
Gene ID (Entrez)
7161
Immunogen
This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone
5B429
Dilution
Western Blot 2 ug/ml, Simple Western 1:25, Flow Cytometry 1 ug/mL. Use reported in scientific literature (PMID 21152443), Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 5-15 ug/ml, Immunohistochemistry-Frozen 5-15 ug/ml, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:20-1:1000, CyTOF-ready
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat
Gene Alias
p53-related protein, P73p53-like transcription factor, TA p73, TAp73, tumor protein p73
Gene Symbols
TP73
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674/ IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).
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Description
- Description p73 Monoclonal specifically detects p73 in Human, Mouse, Rat samples
- It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-ready.
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Antigen: p73
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Cancer, Tumor Suppressors
Gene Accession No.: O15350
Gene ID (Entrez): 7161
Immunogen: This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 5B429
Dilution: Western Blot 2 ug/ml, Simple Western 1:25, Flow Cytometry 1 ug/mL. Use reported in scientific literature (PMID 21152443), Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 5-15 ug/ml, Immunohistochemistry-Frozen 5-15 ug/ml, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:20-1:1000, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat
Gene Alias: p53-related protein, P73p53-like transcription factor, TA p73, TAp73, tumor protein p73
Gene Symbols: TP73
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674/ IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).
Antigen: PAR1/Thrombin Receptor
Concentration: 1 mg/mL
Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Cancer, GPCR
Gene Accession No.: P25116, P30558
Gene ID (Entrez): 2149
Immunogen: The oligopeptide CNATLDPRSFLL from human Thrombin Receptor. [Swiss-Prot# P25116]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: N2-11
Dilution: Western Blot 1:1000, ELISA 1:100 - 1:2000, Immunohistochemistry 1:100. Use reported in scientific literature (PMID 35055169), Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen reported in scientific literature (PMID 31885791), Immunofluorescence
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse
Gene Alias: CF2R, CF2RHTR, coagulation factor II (thrombin) receptor, Coagulation factor II receptor, HTR, PAR1, PAR1PAR-1protease-activated receptor 1, Protease-Activated Receptor 1, Thrombin receptor, TRproteinase-activated receptor 1
Gene Symbols: F2R
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: __
Antigen: Park7/DJ-1
Concentration: 1 mg/mL
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, KnockDown
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Alzheimers Research, Apoptosis, Cancer, DNA replication Transcription Translation and Splicing, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Signal Transduction, Tumor Suppressors
Gene Accession No.: Q99497
Gene ID (Entrez): 11315
Immunogen: Full length recombinant human Park7(DJ-1) expressed in and purified from E. coli. [UniProt# Q99497]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 4H4
Dilution: Western Blot 1:5000, Simple Western 1:500, Immunohistochemistry 1:5000, Immunocytochemistry/Immunofluorescence 1:5000, Knockout Validated
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Bovine, Mouse (Negative), Rat (Negative)
Gene Alias: DJ-1, DJ1FLJ34360, EC 3.4, FLJ27376, FLJ92274, Oncogene DJ1, Parkinson disease (autosomal recessive, early onset) 7, Parkinson disease protein 7, protein DJ-1
Gene Symbols: PARK7
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: Human, Bovine and Mouse.
