4976398
His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 5 publications
Manufacturer: Fischer Scientific
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Antigen
His Tag
Concentration
1 mg/mL
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cellular Markers, Epitope Tags
Immunogen
PAX6 transcription factor linked to histidine tag
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone
AD1.1.10
Dilution
Western Blot 1:1000, Simple Western 1:250, Flow Cytometry 1:10 - 1:50, ELISA 1:100 - 1:500, Immunohistochemistry 1:100 - 1:300, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:300, In vitro assay
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Gene Alias
6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, Poly-histidine
Isotype
IgG1
Purification Method
Protein G purified
Test Specificity
This antibody is reactive to histidine tags.
Related Products
Description
- His Tag Monoclonal specifically detects His Tag in Epitope Tag samples
- It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Frozen, In vitro assay.
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Antigen: His Tag
Concentration: 1 mg/mL
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cellular Markers, Epitope Tags
Immunogen: PAX6 transcription factor linked to histidine tag
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: AD1.1.10
Dilution: Western Blot 1:1000, Simple Western 1:250, Flow Cytometry 1:10 - 1:50, ELISA 1:100 - 1:500, Immunohistochemistry 1:100 - 1:300, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:300, In vitro assay
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Gene Alias: 6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, Poly-histidine
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: This antibody is reactive to histidine tags.
Antigen:
His Tag
Concentration:
1 mg/mL
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cellular Markers, Epitope Tags
Immunogen:
PAX6 transcription factor linked to histidine tag
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
AD1.1.10
Dilution:
Western Blot 1:1000, Simple Western 1:250, Flow Cytometry 1:10 - 1:50, ELISA 1:100 - 1:500, Immunohistochemistry 1:100 - 1:300, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:300, In vitro assay
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Gene Alias:
6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, Poly-histidine
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
This antibody is reactive to histidine tags.
Antigen: Desmoglein-3
Concentration: 1 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cytoskeleton Markers, Extracellular Matrix, Signal Transduction
Immunogen: Human Desmoglein 3 recombinant protein [UniProt# P32926]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 5G11
Dilution: Western Blot 1:500 - 1:1000, Flow Cytometry 1 ug per million cells, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:200, Dot Blot reported in scientific literature (PMID 15588331)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Gene Alias: Cadherin family member 6, CDHF6130-kD pemphigus vulgaris antigen, desmoglein 3, desmoglein 3 (pemphigus vulgaris antigen), desmoglein-3, DKFZp686P23184,130 kDa pemphigus vulgaris antigen, pemphigus vulgaris antigen, PVA
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: This antibody reacts with Desmoglein-3. Does not cross-react with Desmoglein 1 or Desmoglein 2.
Antigen:
Desmoglein-3
Concentration:
1 mg/mL
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cytoskeleton Markers, Extracellular Matrix, Signal Transduction
Immunogen:
Human Desmoglein 3 recombinant protein [UniProt# P32926]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
5G11
Dilution:
Western Blot 1:500 - 1:1000, Flow Cytometry 1 ug per million cells, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:200, Dot Blot reported in scientific literature (PMID 15588331)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Gene Alias:
Cadherin family member 6, CDHF6130-kD pemphigus vulgaris antigen, desmoglein 3, desmoglein 3 (pemphigus vulgaris antigen), desmoglein-3, DKFZp686P23184,130 kDa pemphigus vulgaris antigen, pemphigus vulgaris antigen, PVA
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
This antibody reacts with Desmoglein-3. Does not cross-react with Desmoglein 1 or Desmoglein 2.
Antigen: Desmoglein-3
Concentration: 1 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cytoskeleton Markers, Extracellular Matrix, Signal Transduction
Immunogen: Human Desmoglein 3 recombinant protein [UniProt# P32926]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 5G11
Dilution: Western Blot 1:500 - 1:1000, Flow Cytometry 1 ug per million cells, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:200, Dot Blot reported in scientific literature (PMID 15588331)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Gene Alias: Cadherin family member 6, CDHF6130-kD pemphigus vulgaris antigen, desmoglein 3, desmoglein 3 (pemphigus vulgaris antigen), desmoglein-3, DKFZp686P23184,130 kDa pemphigus vulgaris antigen, pemphigus vulgaris antigen, PVA
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: This antibody reacts with Desmoglein-3. Does not cross-react with Desmoglein 1 or Desmoglein 2.
Antigen:
Desmoglein-3
Concentration:
1 mg/mL
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cytoskeleton Markers, Extracellular Matrix, Signal Transduction
Immunogen:
Human Desmoglein 3 recombinant protein [UniProt# P32926]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
5G11
Dilution:
Western Blot 1:500 - 1:1000, Flow Cytometry 1 ug per million cells, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:200, Dot Blot reported in scientific literature (PMID 15588331)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Gene Alias:
Cadherin family member 6, CDHF6130-kD pemphigus vulgaris antigen, desmoglein 3, desmoglein 3 (pemphigus vulgaris antigen), desmoglein-3, DKFZp686P23184,130 kDa pemphigus vulgaris antigen, pemphigus vulgaris antigen, PVA
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
This antibody reacts with Desmoglein-3. Does not cross-react with Desmoglein 1 or Desmoglein 2.
Antigen: ABCA1
Concentration: 1 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: ABC Transporters, Astrocyte Markers, Cancer, Cellular Markers, Cholesterol Metabolism, Glia Markers, Golgi Apparatus Markers, Lipid and Metabolism, Neuroscience, Signal Transduction
Immunogen: 50 kDa N-terminal extracellular loop of mouse ABCA1 [UniProt# P41233]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: HJ1
Dilution: Western Blot 1:2000, Flow Cytometry 1 ug per million cells. Use reported in scientific literature, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 31061090), Immunohistochemistry-Paraffin 1:400, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Gene Alias: ABC-1, ABC1ATP-binding cassette transporter A1, ATP-binding cassette 1, ATP-binding cassette transporter 1, ATP-binding cassette, sub-family A (ABC1), member 1, CERP, Cholesterol efflux regulatory protein, EC 2.7.7.8, EC 2.8.1.8, EC 3.6.3, EC 3.6.3.41, FLJ14958, HDLDT1, membrane-bound, MGC164864, MGC165011, TGDATP-binding cassette sub-family A member 1
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: __
Antigen:
ABCA1
Concentration:
1 mg/mL
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
ABC Transporters, Astrocyte Markers, Cancer, Cellular Markers, Cholesterol Metabolism, Glia Markers, Golgi Apparatus Markers, Lipid and Metabolism, Neuroscience, Signal Transduction
Immunogen:
50 kDa N-terminal extracellular loop of mouse ABCA1 [UniProt# P41233]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
HJ1
Dilution:
Western Blot 1:2000, Flow Cytometry 1 ug per million cells. Use reported in scientific literature, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 31061090), Immunohistochemistry-Paraffin 1:400, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Gene Alias:
ABC-1, ABC1ATP-binding cassette transporter A1, ATP-binding cassette 1, ATP-binding cassette transporter 1, ATP-binding cassette, sub-family A (ABC1), member 1, CERP, Cholesterol efflux regulatory protein, EC 2.7.7.8, EC 2.8.1.8, EC 3.6.3, EC 3.6.3.41, FLJ14958, HDLDT1, membrane-bound, MGC164864, MGC165011, TGDATP-binding cassette sub-family A member 1
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
__