DNMT3A Antibody (64B1446) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
DNMT3A
Concentration
1.0 mg/mL
Applications
Western Blot, ChIP Assay, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Chromatin Modifiers, Chromatin Research, DNA Methyltransferases, DNA Repair, DNA replication Transcription Translation and Splicing, Epigenetics
Gene Accession No.
Q9Y6K1
Gene ID (Entrez)
1788
Immunogen
This DNMT3A antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone
64B1446
Dilution
Western Blot, Chromatin Immunoprecipitation reported in scientific literature (PMID 24623306), Flow Cytometry 1 ug per million cells, Immunohistochemistry reported in multiple pieces of scientific literature, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 ug/ml. Use reported in scientific literature (PMID 22134929), Immunohistochemistry-Frozen reported in scientific literature (PMID 22134929), Immunohistochemistry (Whole-Mount) reported in scientific literature (PMID 26507142), Chromatin Immunoprecipitation (ChIP), CyTOF-ready
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat
Gene Alias
64B1446 clone, DNA (cytosine-5-)-methyltransferase 3 alpha, DNA (cytosine-5)-methyltransferase 3A, DNA cytosine methyltransferase 3A2, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, Dnmt3a, DNMT3A2, EC 2.1.1.37, M.HsaIIIA
Gene Symbols
DNMT3A
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Cross-reacts with Human (weakly) and Mouse. Not yet tested in other species.
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Description
- DNMT3A Monoclonal specifically detects DNMT3A in Human, Mouse, Rat samples
- It is validated for Western Blot, ChIP assay, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunohistochemistry Whole-Mount, CyTOF.