4977050

DNMT1 Antibody (60B1220.1) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 119 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

DNMT1

Concentration

1.0 mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Chromatin Modifiers, Chromatin Research, Core ESC Like Genes, DNA Methyltransferases, Epigenetics, Stem Cell Markers, Transcription Factors and Regulators

Gene Accession No.

P26358

Gene ID (Entrez)

1786

Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone

60B1220.1

Dilution

Western Blot 0.1-0.5 ug/ml, Simple Western 1:100, Flow Cytometry 1 - 5 ug/mL, Immunohistochemistry 1:50-1:500, Immunocytochemistry/Immunofluorescence 1:10. Use reported in scientific literature (PMID 24386225), Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:500, Immunohistochemistry-Frozen 1:500, Flow (Intracellular) reported in scientific literature (PMID 24578214), Immunohistochemistry Free-Floating 1:500, Chromatin Immunoprecipitation (ChIP) 1:20-1:1000, CyTOF-ready, KnockDown Validated reported in scientific literature (PMID 31799621)

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Porcine

Gene Alias

AIM, CXXC9CXXC finger protein 9, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, Dnmt1, EC 2.1.1.37, FLJ16293, m.HsaI, MCMTMGC104992

Gene Symbols

DNMT1

Isotype

IgG1 κ

Purification Method

Protein G purified

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Description

  • DNMT1 Monoclonal specifically detects DNMT1 in Human, Mouse, Rat, Porcine, Bovine, Sheep samples
  • It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Immunohistochemistry Free-Floating, CyTOF-ready, Knockdown Validated.

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Antigen:
DNMT1

Concentration:
1.0 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Chromatin Modifiers, Chromatin Research, Core ESC Like Genes, DNA Methyltransferases, Epigenetics, Stem Cell Markers, Transcription Factors and Regulators

Gene Accession No.:
P26358

Gene ID (Entrez):
1786

Immunogen:
This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone:
60B1220.1

Dilution:
Western Blot 0.1-0.5 ug/ml, Simple Western 1:100, Flow Cytometry 1 - 5 ug/mL, Immunohistochemistry 1:50-1:500, Immunocytochemistry/Immunofluorescence 1:10. Use reported in scientific literature (PMID 24386225), Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:500, Immunohistochemistry-Frozen 1:500, Flow (Intracellular) reported in scientific literature (PMID 24578214), Immunohistochemistry Free-Floating 1:500, Chromatin Immunoprecipitation (ChIP) 1:20-1:1000, CyTOF-ready, KnockDown Validated reported in scientific literature (PMID 31799621)

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine

Gene Alias:
AIM, CXXC9CXXC finger protein 9, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, Dnmt1, EC 2.1.1.37, FLJ16293, m.HsaI, MCMTMGC104992

Gene Symbols:
DNMT1

Isotype:
IgG1 κ

Purification Method:
Protein G purified

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Antigen:
DNMT3A

Concentration:
1.0 mg/mL

Applications:
Western Blot, ChIP Assay, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Chromatin Modifiers, Chromatin Research, DNA Methyltransferases, DNA Repair, DNA replication Transcription Translation and Splicing, Epigenetics

Gene Accession No.:
Q9Y6K1

Gene ID (Entrez):
1788

Immunogen:
This DNMT3A antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone:
64B1446

Dilution:
Western Blot, Chromatin Immunoprecipitation reported in scientific literature (PMID 24623306), Flow Cytometry 1 ug per million cells, Immunohistochemistry reported in multiple pieces of scientific literature, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 ug/ml. Use reported in scientific literature (PMID 22134929), Immunohistochemistry-Frozen reported in scientific literature (PMID 22134929), Immunohistochemistry (Whole-Mount) reported in scientific literature (PMID 26507142), Chromatin Immunoprecipitation (ChIP), CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat

Gene Alias:
64B1446 clone, DNA (cytosine-5-)-methyltransferase 3 alpha, DNA (cytosine-5)-methyltransferase 3A, DNA cytosine methyltransferase 3A2, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, Dnmt3a, DNMT3A2, EC 2.1.1.37, M.HsaIIIA

Gene Symbols:
DNMT3A

Isotype:
IgG1 κ

Purification Method:
Protein G purified

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Antigen:
DNMT3A

Concentration:
0.5 mg/mL

Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Chromatin Modifiers, Chromatin Research, DNA Methyltransferases, DNA Repair, DNA replication Transcription Translation and Splicing, Epigenetics

Gene Accession No.:
Q9Y6K1

Gene ID (Entrez):
1788

Immunogen:
This antibody was raised against bacteria expressed HIS-tag recombinant mouse Dnmt3a.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone:
64B814.1

Dilution:
Western Blot 1-3 ug/ml, Immunocytochemistry/Immunofluorescence 5 ug/ml, Flow (Intracellular)

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
DNA (cytosine-5-)-methyltransferase 3 alpha, DNA (cytosine-5)-methyltransferase 3A, DNA cytosine methyltransferase 3A2, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, Dnmt3a, DNMT3A2, EC 2.1.1.37, M.HsaIIIA

Gene Symbols:
DNMT3A

Isotype:
IgG1 κ

Purification Method:
Protein G purified

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497738

0.5 M in THF...


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