TLR4 Antibody (76B357.1) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
TLR4
Concentration
1.0 mg/mL
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In vitro Assay, KnockDown, Neutralization
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Toll Like Receptors
Gene Accession No.
O00206
Gene ID (Entrez)
7099
Immunogen
This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone
76B357.1
Dilution
Western Blot 1-3 ug/ml, Flow Cytometry, ELISA, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 ug/ml, Immunohistochemistry-Frozen 1:500, In vitro assay, Dot Blot, SDS-Page reported in scientific literature (PMID 33166339), Flow (Cell Surface), Flow (Intracellular), ELISA Capture (Matched Antibody Pair) 0.5 ug, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready, Knockout Validated, KnockDown Validated, Block/Neutralize, Dual RNAscope ISH-IHC
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Porcine, Bovine
Gene Alias
76B357.1, ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, tlr4 76B357.1, TLR4 facs, TLR4 flow cytometry, TLR4 human, TLR4 ihc, TLR4 western blot, TOLL, toll-like receptor 4
Gene Symbols
TLR4
Isotype
IgG2b κ
Purification Method
Protein G purified
Test Specificity
The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse. Human, Mouse, Rat. Ground squirrel reactivity reported by a customer review. Predicted cross-reactivity based on sequence identity: Chimp (100%), Baboon (100%), Chinese Hamster (100%), Bovine (93%), Pig (93%). Mammal reactivity reported in scientific literature (PMID: 25130694).
Related Products
Description
- TLR4 Monoclonal specifically detects TLR4 in Human, Mouse, Rat, Porcine, Bovine, Mammal samples
- It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, In vitro assay, Dot Blot, SDS-Page, Flow (Cell Surface), Flow (Intracellular), ELISA Capture (Matched Antibody Pair), CyTOF-ready, Knockout Validated, Knockdown Validated, Block/Neutralize, Dual RNAscope ISH-IHC.
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Antigen: TLR4
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In vitro Assay, KnockDown, Neutralization
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Toll Like Receptors
Gene Accession No.: O00206
Gene ID (Entrez): 7099
Immunogen: This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 76B357.1
Dilution: Western Blot 1-3 ug/ml, Flow Cytometry, ELISA, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 ug/ml, Immunohistochemistry-Frozen 1:500, In vitro assay, Dot Blot, SDS-Page reported in scientific literature (PMID 33166339), Flow (Cell Surface), Flow (Intracellular), ELISA Capture (Matched Antibody Pair) 0.5 ug, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready, Knockout Validated, KnockDown Validated, Block/Neutralize, Dual RNAscope ISH-IHC
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine
Gene Alias: 76B357.1, ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, tlr4 76B357.1, TLR4 facs, TLR4 flow cytometry, TLR4 human, TLR4 ihc, TLR4 western blot, TOLL, toll-like receptor 4
Gene Symbols: TLR4
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse. Human, Mouse, Rat. Ground squirrel reactivity reported by a customer review. Predicted cross-reactivity based on sequence identity: Chimp (100%), Baboon (100%), Chinese Hamster (100%), Bovine (93%), Pig (93%). Mammal reactivity reported in scientific literature (PMID: 25130694).
Antigen: __
Concentration: __
Applications: __
Conjugate: __
Host Species: __
Research Discipline: __
Gene Accession No.: __
Gene ID (Entrez): __
Immunogen: __
Primary or Secondary: __
Content And Storage: __
Clone: __
Dilution: __
Classification: __
Form: __
Regulatory Status: __
Target Species: __
Gene Alias: __
Gene Symbols: __
Isotype: __
Purification Method: __
Test Specificity: __
Antigen: TLR6
Concentration: 1.0 mg/ml
Applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Immunology, Innate Immunity, Prostate Cancer, Toll Like Receptors
Gene Accession No.: Q9Y2C9
Gene ID (Entrez): 10333
Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids 408-424 of human TRL6.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 86B1153.2
Dilution: Western Blot reported in scientific literature (PMID 17332440), Flow Cytometry 3 ug/10-6 cells, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 5 ug/ml, Flow (Cell Surface) reported in scientific literature (PMID 24986635), Flow (Intracellular)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD286, CD286 antigen, toll-like receptor 6
Gene Symbols: TLR6
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: TLR6 (86B1153.2)
