HIF-1 alpha Antibody (H1alpha67), Novus Biologicals™
Mouse Monoclonal Antibody has been used in 891 publication
Manufacturer: Fischer Scientific
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Antigen
HIF-1 alpha
Concentration
1.0 mg/mL
Applications
Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, In vitro Assay, KnockDown
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
Gene Accession No.
Q16665
Gene ID (Entrez)
3091
Immunogen
This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
93 kDa
Clone
H1alpha67
Dilution
Western Blot 1:500, Simple Western, Chromatin Immunoprecipitation 1 - 5 ug/IP. Use reported in scientific literature, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 20042684), Immunohistochemistry 1:20-1:50, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:20-1:50, Immunohistochemistry-Frozen 1:20-1:50, Immunoassay reported in scientific literature (PMID 26147748), Immunoblotting reported in multiple pieces of scientific literature, In vitro assay reported in multiple pieces of scientific literature, Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature (PMID 22411794), Proximity Ligation Assay reported in scientific literature (PMID 27595394), Tissue Culture Substratum, Ligand Activation reported in scientific literature (PMID 26147748), Immunohistochemistry Free-Floating reported in scientific literature (PMID 33242463), Chromatin Immunoprecipitation (ChIP)
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Porcine, Bovine, Canine, Feline, Ferret, Monkey, Primate, Rabbit, Sheep, Xenopus species
Gene Alias
AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1
Gene Symbols
HIF1A
Isotype
IgG2b
Purification Method
Protein G purified
Test Specificity
Reactivity withCandida albicansreported by a customer review. Reactivity with Xenopus reported in the scientific literature (PMID: 18303027).
Related Products
Description
- HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Porcine, Bovine, Canine, Feline, Ferret, Monkey, Primate, Rabbit, Sheep, Xenopus samples
- It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoassay, Immunoblotting, In vitro assay, Gel Super Shift Assays, Proximity Ligation Assay, Tissue Culture Substratum, Ligand Activation, Immunohistochemistry Free-Floating, Knockout Validated, Knockdown Validated.
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Show Difference
Antigen: HIF-1 alpha
Concentration: 1.0 mg/mL
Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, In vitro Assay, KnockDown
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
Gene Accession No.: Q16665
Gene ID (Entrez): 3091
Immunogen: This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 93 kDa
Clone: H1alpha67
Dilution: Western Blot 1:500, Simple Western, Chromatin Immunoprecipitation 1 - 5 ug/IP. Use reported in scientific literature, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 20042684), Immunohistochemistry 1:20-1:50, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:20-1:50, Immunohistochemistry-Frozen 1:20-1:50, Immunoassay reported in scientific literature (PMID 26147748), Immunoblotting reported in multiple pieces of scientific literature, In vitro assay reported in multiple pieces of scientific literature, Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature (PMID 22411794), Proximity Ligation Assay reported in scientific literature (PMID 27595394), Tissue Culture Substratum, Ligand Activation reported in scientific literature (PMID 26147748), Immunohistochemistry Free-Floating reported in scientific literature (PMID 33242463), Chromatin Immunoprecipitation (ChIP)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine, Canine, Feline, Ferret, Monkey, Primate, Rabbit, Sheep, Xenopus species
Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1
Gene Symbols: HIF1A
Isotype: IgG2b
Purification Method: Protein G purified
Test Specificity: Reactivity withCandida albicansreported by a customer review. Reactivity with Xenopus reported in the scientific literature (PMID: 18303027).
Antigen: HIP1
Concentration: __
Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neurodegeneration, Neuronal Cell Markers, Neuroscience
Gene Accession No.: O00291
Gene ID (Entrez): 3092
Immunogen: Human 3'-HIP1 (∼65 kDa)
Primary or Secondary: Primary
Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 115 kDa
Clone: 1B11
Dilution: Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation reported in scientific literature (PMID 23272104)
Classification: Monoclonal
Form: Ascites
Regulatory Status: RUO
Target Species: Human, Mouse, Bacteria, Rat (Negative)
Gene Alias: HIP-1, HIP-I, huntingtin interacting protein 1, huntingtin-interacting protein 1, Huntingtin-interacting protein I, ILWEQ, MGC126506
Gene Symbols: HIP1
Isotype: IgG1 κ
Purification Method: Unpurified
Test Specificity: NB300-204 is specific for human HIP1. Does not cross-react with HIP1r.
Antigen: Histone H3 (p Ser28)
Concentration: 0.5 mg/mL
Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Chromatin Modifiers, Chromatin Research, Epigenetics, Histones and Modified Histones, Loading Controls
Gene Accession No.: Q71DI3
Gene ID (Entrez): 126961
Immunogen: This Histone H3 [p Ser28] antibody (117C826) was raised against a synthetic peptide containing phospho serine at position 28 CARKS*APATGGVKK of human Histone H3. The immunogen sequence is conserved across mammalian and invertebrates, as well as plants.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 15 kDa
Clone: 117C826
Dilution: Western Blot 1-2 ug/ml, Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (PMID 22279221), Immunohistochemistry-Paraffin reported in scientific literature (PMID 35897708)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: H3, H3 histone, family 3A, H3.3A, H3.3B, H3F2, H3F3, H3F3B, H3FM, H3S28p, HIST2H3C, histone H3.3, MGC87782, MGC87783
Gene Symbols: H3C14
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: In anti-Fas mAb treated Jurkat, an 18 kDa band is observed using Histone H3 [p Ser28] antibody (117C826).
