4978557
Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 11 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Alkaline Phosphatase/ALPP
Concentration
1.0 mg/mL
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Embryonic Stem Cell Markers, Ovarian Carcinoma Cell Markers, Stem Cell Markers
Gene Accession No.
P05187
Gene ID (Entrez)
250
Immunogen
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
70 kDa
Clone
8B6
Dilution
Western Blot 1:1000, Flow Cytometry reported in multiple pieces of scientific literature, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:200, Immunohistochemistry-Frozen 1:10-1:500, Radioimmunodiffusion
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat
Gene Alias
Alkaline phosphatase Regan isozyme, alkaline phosphatase, placental, alkaline phosphatase, placental (Regan isozyme), alkaline phosphatase, placental type, alkaline phosphomonoesterase, ALP, EC 3.1.3.1, FLJ61142, glycerophosphatase, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1, Regan isozyme
Gene Symbols
ALPP
Isotype
IgG2a κ
Purification Method
Protein A purified
Test Specificity
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
Related Products
Description
- Alkaline Phosphatase/ALPP Monoclonal specifically detects Alkaline Phosphatase/ALPP in Human, Mouse, Rat samples
- It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Radioimmunodiffusion.
Compare Similar Items
Show Difference
Antigen: Alkaline Phosphatase/ALPP
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Embryonic Stem Cell Markers, Ovarian Carcinoma Cell Markers, Stem Cell Markers
Gene Accession No.: P05187
Gene ID (Entrez): 250
Immunogen: Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 70 kDa
Clone: 8B6
Dilution: Western Blot 1:1000, Flow Cytometry reported in multiple pieces of scientific literature, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:200, Immunohistochemistry-Frozen 1:10-1:500, Radioimmunodiffusion
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat
Gene Alias: Alkaline phosphatase Regan isozyme, alkaline phosphatase, placental, alkaline phosphatase, placental (Regan isozyme), alkaline phosphatase, placental type, alkaline phosphomonoesterase, ALP, EC 3.1.3.1, FLJ61142, glycerophosphatase, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1, Regan isozyme
Gene Symbols: ALPP
Isotype: IgG2a κ
Purification Method: Protein A purified
Test Specificity: Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
Antigen:
Alkaline Phosphatase/ALPP
Concentration:
1.0 mg/mL
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cancer, Embryonic Stem Cell Markers, Ovarian Carcinoma Cell Markers, Stem Cell Markers
Gene Accession No.:
P05187
Gene ID (Entrez):
250
Immunogen:
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
70 kDa
Clone:
8B6
Dilution:
Western Blot 1:1000, Flow Cytometry reported in multiple pieces of scientific literature, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:200, Immunohistochemistry-Frozen 1:10-1:500, Radioimmunodiffusion
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat
Gene Alias:
Alkaline phosphatase Regan isozyme, alkaline phosphatase, placental, alkaline phosphatase, placental (Regan isozyme), alkaline phosphatase, placental type, alkaline phosphomonoesterase, ALP, EC 3.1.3.1, FLJ61142, glycerophosphatase, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1, Regan isozyme
Gene Symbols:
ALPP
Isotype:
IgG2a κ
Purification Method:
Protein A purified
Test Specificity:
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
Antigen: GAPDH
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Alzheimers Research, Apoptosis, Autophagy, Cancer, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
Gene Accession No.: P04406
Gene ID (Entrez): 2597
Immunogen: GAPDH antibody developed against purified recombinant fragment of human GAPDH expressed in E. coli. [UniProt# P04406]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 36 kDa
Clone: 1A10
Dilution: Western Blot 1:500-1:2000, Simple Western 1:50, Flow Cytometry 5 ug/ml, ELISA 1:10000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunohistochemistry-Paraffin 1:200-1:1000
Classification: Monoclonal
Form: Precipitation
Regulatory Status: RUO
Target Species: Human, Mouse
Gene Alias: aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
Gene Symbols: GAPDH
Isotype: IgG1
Purification Method: Ammonium sulfate precipitation
Test Specificity: __
Antigen:
GAPDH
Concentration:
1.0 mg/mL
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Alzheimers Research, Apoptosis, Autophagy, Cancer, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
Gene Accession No.:
P04406
Gene ID (Entrez):
2597
Immunogen:
GAPDH antibody developed against purified recombinant fragment of human GAPDH expressed in E. coli. [UniProt# P04406]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
36 kDa
Clone:
1A10
Dilution:
Western Blot 1:500-1:2000, Simple Western 1:50, Flow Cytometry 5 ug/ml, ELISA 1:10000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunohistochemistry-Paraffin 1:200-1:1000
Classification:
Monoclonal
Form:
Precipitation
Regulatory Status:
RUO
Target Species:
Human, Mouse
Gene Alias:
aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
Gene Symbols:
GAPDH
Isotype:
IgG1
Purification Method:
Ammonium sulfate precipitation
Test Specificity:
__
Antigen: GAPDH
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Alzheimers Research, Apoptosis, Autophagy, Cancer, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
Gene Accession No.: P04406
Gene ID (Entrez): 2597
Immunogen: GAPDH antibody developed against purified recombinant fragment of human GAPDH expressed in E. coli. [UniProt# P04406]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 36 kDa
Clone: 1A10
Dilution: Western Blot 1:500-1:2000, Simple Western 1:50, Flow Cytometry 5 ug/ml, ELISA 1:10000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunohistochemistry-Paraffin 1:200-1:1000
Classification: Monoclonal
Form: Precipitation
Regulatory Status: RUO
Target Species: Human, Mouse
Gene Alias: aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
Gene Symbols: GAPDH
Isotype: IgG1
Purification Method: Ammonium sulfate precipitation
Test Specificity: __
Antigen:
GAPDH
Concentration:
1.0 mg/mL
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Alzheimers Research, Apoptosis, Autophagy, Cancer, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
Gene Accession No.:
P04406
Gene ID (Entrez):
2597
Immunogen:
GAPDH antibody developed against purified recombinant fragment of human GAPDH expressed in E. coli. [UniProt# P04406]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
36 kDa
Clone:
1A10
Dilution:
Western Blot 1:500-1:2000, Simple Western 1:50, Flow Cytometry 5 ug/ml, ELISA 1:10000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunohistochemistry-Paraffin 1:200-1:1000
Classification:
Monoclonal
Form:
Precipitation
Regulatory Status:
RUO
Target Species:
Human, Mouse
Gene Alias:
aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
Gene Symbols:
GAPDH
Isotype:
IgG1
Purification Method:
Ammonium sulfate precipitation
Test Specificity:
__
Antigen: GAPDH
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Alzheimers Research, Apoptosis, Autophagy, Cancer, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
Gene Accession No.: P00355
Gene ID (Entrez): 2597
Immunogen: This GAPDH antibody was developed against full length purified porcine GAPDH [UniProt# P00355]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 36 kDa
Clone: 1D4
Dilution: Western Blot 1:1000, Simple Western 1:25, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Single Cell Western, KnockDown Validated
Classification: Monoclonal
Form: Supernatant
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Equine, Feline, Hamster, Opossum, Primate, Rabbit, Sheep, Zebrafish
Gene Alias: 1D4 GAPDH, aging-associated gene 9 protein, Cytoplasm Marker, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, GAPDPeptidyl-cysteine S-nitrosylase GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38, OCT1 coactivator in S phase, Peptidyl-cysteine S-nitrosylase GAPDH
Gene Symbols: GAPDH
Isotype: IgM
Purification Method: Immunogen affinity purified
Test Specificity: Human, mouse, rat, bovine, porcine, avian, sheep, opossum and primate. Likely to react with other mammals as well. Hamster and zebrafish reactivity reported by a customer review. Chicken reactivity reported in scientific literature (PMID: 24725420).
Antigen:
GAPDH
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Alzheimers Research, Apoptosis, Autophagy, Cancer, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
Gene Accession No.:
P00355
Gene ID (Entrez):
2597
Immunogen:
This GAPDH antibody was developed against full length purified porcine GAPDH [UniProt# P00355]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
36 kDa
Clone:
1D4
Dilution:
Western Blot 1:1000, Simple Western 1:25, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Single Cell Western, KnockDown Validated
Classification:
Monoclonal
Form:
Supernatant
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Equine, Feline, Hamster, Opossum, Primate, Rabbit, Sheep, Zebrafish
Gene Alias:
1D4 GAPDH, aging-associated gene 9 protein, Cytoplasm Marker, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, GAPDPeptidyl-cysteine S-nitrosylase GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38, OCT1 coactivator in S phase, Peptidyl-cysteine S-nitrosylase GAPDH
Gene Symbols:
GAPDH
Isotype:
IgM
Purification Method:
Immunogen affinity purified
Test Specificity:
Human, mouse, rat, bovine, porcine, avian, sheep, opossum and primate. Likely to react with other mammals as well. Hamster and zebrafish reactivity reported by a customer review. Chicken reactivity reported in scientific literature (PMID: 24725420).