Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Research Discipline:
Cancer, Cell Biology, Cell Cycle and Replication, Chromatin Research, p53 Pathway, Transcription Factors and Regulators, Tumor Suppressors
Formulation:
Ascites with 0.1% Sodium Azide
Immunogen:
A synthetic peptide made to a sequence (within residues 1-100) of mouse p19ARF. [UniProt# Q64364]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Dilution:
Western Blot 1:500, ELISA 1:5000-1:10000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification:
Monoclonal
Target Species:
Mouse, Hamster (Negative), Human (Negative)
Gene Alias:
ARF, CDK4 inhibitor p16-INK4, CDK4IP14ARF, CDKN2, cell cycle negative regulator beta, CMM2P16-INK4A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4), INK4, INK4A, MLMP16INK4, MTS-1, MTS1P14, Multiple tumor suppressor 1, p14, p14ARF, p16, p16-INK4, p16INK4a, p16-INK4a, P19, p19ARF, TP16
Purification Method:
Unpurified
Test Specificity:
This antibody is specific for murine p19ARF. It does not recognize human p14Arf nor Golden hamster p13Arf.