4986675
O-GlcNAc Antibody (HGAC85), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
O-GlcNAc
Dilution
Western Blot 1:500-1:1000, Chromatin Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 20368426), ELISA 1:100-1:2000, Immunohistochemistry 1:200. Use reported in scientific literature (Zhang, et al), Immunocytochemistry/Immunofluorescence 1:100 - 1:200. Use reported in scientific literature (Hamiel CR et al), Immunoprecipitation 1:10-1:500. Use reported in scientific literature (Hamiel CR et al), Immunohistochemistry-Paraffin 1:200, Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Classification
Monoclonal
Form
Ascites
Regulatory Status
RUO
Target Species
Human, All species, Primate
Gene Alias
GlcNAc, O-linked N-acetylglucosamine
Isotype
IgG3 κ
Purification Method
Unpurified
Test Specificity
Recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins
Clone
HGAC85
Applications
Western Blot, ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction
Formulation
Ascites with 0.1% Sodium Azide
Immunogen
Heat-killed, pepsin-treated group A streptococci (Streptococcus pyogenes)
Primary or Secondary
Primary
Content And Storage
Store at -20C. Avoid freeze-thaw cycles.
Related Products
Description
- O-GlcNAc Monoclonal specifically detects O-GlcNAc in Human, All Species, Primate samples
- It is validated for Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.
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Antigen: O-GlcNAc
Dilution: Western Blot 1:500-1:1000, Chromatin Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 20368426), ELISA 1:100-1:2000, Immunohistochemistry 1:200. Use reported in scientific literature (Zhang, et al), Immunocytochemistry/Immunofluorescence 1:100 - 1:200. Use reported in scientific literature (Hamiel CR et al), Immunoprecipitation 1:10-1:500. Use reported in scientific literature (Hamiel CR et al), Immunohistochemistry-Paraffin 1:200, Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Classification: Monoclonal
Form: Ascites
Regulatory Status: RUO
Target Species: Human, All species, Primate
Gene Alias: GlcNAc, O-linked N-acetylglucosamine
Isotype: IgG3 κ
Purification Method: Unpurified
Test Specificity: Recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins
Clone: HGAC85
Applications: Western Blot, ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction
Formulation: Ascites with 0.1% Sodium Azide
Immunogen: Heat-killed, pepsin-treated group A streptococci (Streptococcus pyogenes)
Primary or Secondary: Primary
Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
Antigen:
O-GlcNAc
Dilution:
Western Blot 1:500-1:1000, Chromatin Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 20368426), ELISA 1:100-1:2000, Immunohistochemistry 1:200. Use reported in scientific literature (Zhang, et al), Immunocytochemistry/Immunofluorescence 1:100 - 1:200. Use reported in scientific literature (Hamiel CR et al), Immunoprecipitation 1:10-1:500. Use reported in scientific literature (Hamiel CR et al), Immunohistochemistry-Paraffin 1:200, Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Classification:
Monoclonal
Form:
Ascites
Regulatory Status:
RUO
Target Species:
Human, All species, Primate
Gene Alias:
GlcNAc, O-linked N-acetylglucosamine
Isotype:
IgG3 κ
Purification Method:
Unpurified
Test Specificity:
Recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins
Clone:
HGAC85
Applications:
Western Blot, ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction
Formulation:
Ascites with 0.1% Sodium Azide
Immunogen:
Heat-killed, pepsin-treated group A streptococci (Streptococcus pyogenes)
Primary or Secondary:
Primary
Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.
Antigen: O-GlcNAc
Dilution: Western Blot 1:1000, Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 20404350), Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 12029848), Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:200, Dot Blot 1:800, Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine, Drosophila, Fish, Hamster, Primate, Virus, Xenopus species
Gene Alias: GlcNAc, O-linked N-acetylglucosamine
Isotype: IgG1
Purification Method: Protein A purified
Test Specificity: Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues.
Clone: RL2
Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: __
Formulation: __
Immunogen: Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Primary or Secondary: Primary
Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
Antigen:
O-GlcNAc
Dilution:
Western Blot 1:1000, Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 20404350), Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 12029848), Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:200, Dot Blot 1:800, Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Drosophila, Fish, Hamster, Primate, Virus, Xenopus species
Gene Alias:
GlcNAc, O-linked N-acetylglucosamine
Isotype:
IgG1
Purification Method:
Protein A purified
Test Specificity:
Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues.
Clone:
RL2
Applications:
Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Formulation:
__
Immunogen:
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Primary or Secondary:
Primary
Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.
Antigen: CD45
Dilution: __
Classification: Monoclonal
Form: __
Regulatory Status: RUO
Target Species: Human
Gene Alias: __
Isotype: IgG1 κ
Purification Method: Affinity Purified
Test Specificity: __
Clone: HI30
Applications: Flow Cytometry
Conjugate: Brilliant Violet 711
Host Species: Mouse
Research Discipline: __
Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide
Immunogen: Human Peripheral Blood Leucocytes
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen:
CD45
Dilution:
__
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
__
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Test Specificity:
__
Clone:
HI30
Applications:
Flow Cytometry
Conjugate:
Brilliant Violet 711
Host Species:
Mouse
Research Discipline:
__
Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide
Immunogen:
Human Peripheral Blood Leucocytes
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Antigen: Osteopontin/OPN
Dilution: Western Blot 1:1000, Simple Western 1:20, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Rabbit
Gene Alias: BNSP, Bone sialoprotein 1, MGC110940, Nephropontin, osteopontin, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, uropontin
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: __
Clone: 1B20
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Cancer, Cellular Markers, Hypoxia
Formulation: __
Immunogen: Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
Osteopontin/OPN
Dilution:
Western Blot 1:1000, Simple Western 1:20, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Rabbit
Gene Alias:
BNSP, Bone sialoprotein 1, MGC110940, Nephropontin, osteopontin, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, uropontin
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
__
Clone:
1B20
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis, Cancer, Cellular Markers, Hypoxia
Formulation:
__
Immunogen:
Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.