SIGLEC H Monoclonal Antibody (eBio440c), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse
• Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses
• Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif)
• To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction
• The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG)
• It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6
• Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse
• Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses
• Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif)
• To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction
• The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG)
• It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.