CD3 Monoclonal Antibody (HIT3a), PE, eBioscience™, Invitrogen™

Description

• Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex
• Along with the other CD3 subunits gamma and delta, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex
• CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells
• Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation
• Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HIT3a antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 µL (0.125 µg) per test
• A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex
• T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta
• These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11
• The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits
• CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation
• The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum
• It is never expressed on B or NK cells
• Decreased percentages of T lymphocytes may be observed in some autoimmune diseases
• The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11
• Defects in the CD3 gene are associated with CD3 immunodeficiency.