CD8b Monoclonal Antibody (eBio341 (341)), PE, eBioscience™, Invitrogen™

Description

• Description: The eBio341 monoclonal antibody reacts with rat CD8beta
• CD8beta is a member of the immunoglobulin superfamily which heterodimerizes with CD8alpha to form the CD8 antigen expressed on CD4+CD8+, double-positive thymocytes, the majority of MHC class I-restricted peripheral T cells, and on some macrophages
• CD8beta expression is absent in athymic mice, which are populated by T-cells expressing CD8alpha homodimers
• This has led to the idea that CD8beta expression is dependent on the development of T cells in the thymus
• CD8 binds to MHC class I expressed on the surface of antigen-presenting cells during antigen presentation, and participates in T-cell receptor signal transduction through association with the kinase Lck
• Applications Reported: This eBio341 (341) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBio341 (341) antibody has been tested by flow cytometric analysis of rat splenocytes and thymocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0
• The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system
• The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules
• The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain
• Both alpha and beta chains share significant homology to immunoglobulin variable light chains
• This gene encodes the CD8 beta chain isoforms
• Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described
• A pseudogene, also located on chromosome 2, has been identified.