CD106 (VCAM-1) Monoclonal Antibody (STA), PE, eBioscience™, Invitrogen™

Description

• Description: The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells
• Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation
• CD106 binds to Integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1)
• Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response
• Applications Reported: STA has been reported for use in flow cytometric analysis
• Applications Tested: This STA antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HUVEC
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium
• The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.