50-105-7546
6X His Epitope Tag Monoclonal Antibody (33D10.D2.G8), Rockland™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-105-7546-Each-of-1 | In Stock | ₹ 34,805.81 |
50-105-7546 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 34,805.81
GST (18%): ₹ 6,265.046
Total Price: ₹ 41,070.856
Antigen
6X His Epitope Tag
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG1 κ
Applications
ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Clone
33D10.D2.G8
Conjugate
Unconjugated
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Immunogen
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status
RUO
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Target Molecule
Tag
Related Products
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- Directed against the 6X His motif and is useful in determining its presence in various assays
- This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag
- To date, this antibody has reacted with all His tagged proteins so far tested
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Compare Similar Items
Show Difference
Antigen: 6X His Epitope Tag
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Clone: 33D10.D2.G8
Conjugate: Unconjugated
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Tag
Antigen:
6X His Epitope Tag
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Clone:
33D10.D2.G8
Conjugate:
Unconjugated
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Immunogen:
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Tag
Antigen: Mesothelin
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a
Applications: ELISA, Immunohistochemistry, Western Blot
Clone: MN-1
Conjugate: Unconjugated
Gene Alias: CAK1; CAK1 antigen; megakaryocyte potentiating factor; Megakaryocyte-potentiating factor; Mes; Mesothelin; Mesothelin, cleaved form; Mpf; Msln; Pre-pro-megakaryocyte-potentiating factor; SMR; SMRP; soluble MPF mesothelin related protein
Immunogen: This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Mesothelin
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a
Applications:
ELISA, Immunohistochemistry, Western Blot
Clone:
MN-1
Conjugate:
Unconjugated
Gene Alias:
CAK1; CAK1 antigen; megakaryocyte potentiating factor; Megakaryocyte-potentiating factor; Mes; Mesothelin; Mesothelin, cleaved form; Mpf; Msln; Pre-pro-megakaryocyte-potentiating factor; SMR; SMRP; soluble MPF mesothelin related protein
Immunogen:
This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__
Antigen: CD117 (c-Kit)
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 25 Tests
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: Flow Cytometry
Clone: 104D2
Conjugate: PE
Gene Alias: belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W
Immunogen: __
Regulatory Status: RUO
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Target Molecule: __
Antigen:
CD117 (c-Kit)
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
25 Tests
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Clone:
104D2
Conjugate:
PE
Gene Alias:
belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W
Immunogen:
__
Regulatory Status:
RUO
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Target Molecule:
__
Antigen: Lysozyme
Classification: Polyclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Isotype: __
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: __
Conjugate: Unconjugated
Gene Alias: 1,4-beta-N-acetylmuramidase C; 1700038F02Rik; AI326280; bA534G20.1; c-type lysozyme; KAAG648; LYC2; Lys; Lysm; lysozyme; lysozyme (renal amyloidosis); lysozyme 1; lysozyme 2; Lysozyme C; Lysozyme C type M; Lysozyme C type P; Lysozyme C-1; Lysozyme C-2; lysozyme C-3; lysozyme d1; lysozyme F1; lysozyme like 1; lysozyme-like 1; Lysozyme-like protein 1; lysozyme-like protein 2; Lysz; Lyz; Lyz1; Lyz2; LYZD1; LYZF1; Lyzl1; Lyzs; LZM; Lzm-s1; Lzp; Lzp-s; P lysozyme structural; PRO1278; renal amyloidosis; RGD1559869; UNQ648/PRO1278
Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Lysozyme
Classification:
Polyclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
__
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
__
Conjugate:
Unconjugated
Gene Alias:
1,4-beta-N-acetylmuramidase C; 1700038F02Rik; AI326280; bA534G20.1; c-type lysozyme; KAAG648; LYC2; Lys; Lysm; lysozyme; lysozyme (renal amyloidosis); lysozyme 1; lysozyme 2; Lysozyme C; Lysozyme C type M; Lysozyme C type P; Lysozyme C-1; Lysozyme C-2; lysozyme C-3; lysozyme d1; lysozyme F1; lysozyme like 1; lysozyme-like 1; Lysozyme-like protein 1; lysozyme-like protein 2; Lysz; Lyz; Lyz1; Lyz2; LYZD1; LYZF1; Lyzl1; Lyzs; LZM; Lzm-s1; Lzp; Lzp-s; P lysozyme structural; PRO1278; renal amyloidosis; RGD1559869; UNQ648/PRO1278
Immunogen:
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__