Collagen Type I Polyclonal Antibody, Rockland™

Description

• Store vial at 4°C prior to opening
• This product is stable at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards
• Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues
• This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens
• Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible
• Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain
• Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon
• Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome
• Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity
• Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor
• Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.