CD305 (LAIR1) Monoclonal Antibody (113), PE, eBioscience™, Invitrogen™

Description

• Description: CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system
• The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells
• Unlike human B cells , mouse B cells do not express LAIR-1
• The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase
• Applications Reported: This 113 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 113 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• LAIR1 (CD305) functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells
• Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11
• It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation
• May also play its inhibitory role independently of SH2-containing phosphatases
• Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta
• Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion
• Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells
• Inhibits the differentiation of peripheral blood precursors towards dendritic cells.